Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Development of a Natural Tubular Scaffold From Decellularized Parsley Stems To Be Used in Vascular Tissue Engineering Applications(01. Izmir Institute of Technology, 2024) Çevik, Merve; Dikici, Serkan; Özçivici, Engin; Dikici, Serkan; Özçivici, Engin; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyCardiovascular diseases (CVD) are usually associated with narrowing or blockage of blood vessels and are the leading cause of death globally. By 2030, the annual incidence of CVD-related deaths is estimated to increase 23.3 million. Considering the advancements in endovascular surgery, the use of vascular grafts in cardiovascular surgery is becoming increasingly common. Autografts are the gold standard but have limitations, including limited tissue availability and complications from vessel isolation. Recently, synthetic grafts have emerged as alternatives, though they often fail due to thrombosis, atherosclerosis, intimal hyperplasia, or infection. Thrombosis, the main cause of post-implantation failure, is associated with damage or absence of the endothelial cell lining on the luminal surface of the vascular graft. To overcome the limitations mentioned so far, tissue-engineered vascular grafts (TEVG) have come into prominence. The use of decellularized plant tissues in tissue engineering applications has recently gained great importance. Accordingly, in this study, we fabricated tubular scaffolds from decellularized parsley stems and evaluated them in vitro as potential TEVGs. Our results demonstrated that native plant DNA was successfully removed, and biocompatible tubular biomaterials were successfully fabricated via chemical decellularization of parsley stems. The decellularized parsley stems showed suitable mechanical and biological properties for use as TEVG material. Finally, they were found to provide a convenient environment to form a pseudo-endothelium by recellularization with human endothelial cells prior to implantation. This study is the pioneer in the literature that reports on the potential of parsley stems to be used as a potential TEVG biomaterial.Master Thesis Molecular Characterization of Adult Stem Cells' Adaptations To Mechanical Signals During Adipogenic Commitment(Izmir Institute of Technology, 2015) Özçivici, Engin; Baskan, Öznur; Baskan, Öznur; Yalçın Özuysal, Özden; Özçivici, Engin; Yalçın, Özden; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03. Faculty of Engineering; 04. Faculty of SciencePrevalence of obesity have increased across the years based on technological developments that supported nutritional availability and sedentary lifestyles. Restoring mechanical activity with physical exercise suppresses obesity, and mechanical loading can also be delivered passively with whole body vibrations with high frequency and low magnitude. Anabolic effects of high frequency low magnitude mechanical vibrations on adult stem cells are well identified whereas sensing mechanism of cells and their response to mechanical stimuli is largely unknown. Here, we hypothesed that daily bouts of low intensity vibrations will affect molecular, physical and ultrastructural profile of the cells and the effect will interact with the adipogenic induction. To test this hypothesis mouse bone marrow stem cell line D1 ORL UVA were subjected to mechanical vibrations (0.15g, 90 Hz, 15min/d) for 7 days to both during quiescence and adipogenic commitment. Ultrastructural changes were identified on cellular and molecular levels. Atomic force microscopy was used to characterize the changes on cell surface and significant increase was observed in cell surface height. Moreover, in order to identify the changes in cytoskeleton structure and physical properties, actin were stained with phalloidin and imaged with inverted microscope. To quantify phalloidin amount, signal intensities and physical features of the cells were measured. It was observed that mechanical stimulation and adipogenic induction affect actin content and the physical structure of the cells significantly. Molecular level analysis of cytoskeleton elements and adipogenic markers were performed with Real time PCR. Dramatic increases in adipogenic markers were detected with adipogenic induction. These results indicate that mesenchymal stem cells responds to mechanical vibrations by altering their molecular and ultrastructure during both quiescence and adipogenesis.