Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Magnetic Manipulation of Gravitational Force During Cell Culture(01. Izmir Institute of Technology, 2022) Özçivici, Engin; Özçivici, Engin; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyWith the development of space technologies, one of the most important problems that astronauts will encounter in the planned manned space travels to the Moon and Mars in the near future is the reduced (<1g) gravitational force. Knowledge of the biological effects of partial gravitational force on long-duration space missions is limited. Therefore, it is important to investigate effects of partial gravitational force on physiological adaptation mechanisms. However, methodology to induce partial gravity is expensive and subjected to ethical constraints, therefore there is a need for new ground-based simulation platforms that are able to mimic the partial gravitational force. In this master's thesis, a new partial gravity platform was developed that manipulates gravitational force during cell culture using magnetic fields. First, the platform system was designed and fabricated, and then detailed protocols were described for its use in cell culture. In addition, the effect of simulated partial gravity on cell viability and morphology at the cellular level was examined. The results show that the simulated partial gravity of Mars and the Moon affects the viability and morphology of cells. This new low-cost and easy-to-use partial gravity platform can be used as a ground-based simulation system for gravitational space biology research at the cellular and molecular levels.Master Thesis Cell Patterning With Magnetic Manipulation(01. Izmir Institute of Technology, 2020) Özçivici, Engin; Özçivici, Engin; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyTissue engineering is a biomedical engineering field that provides solutions to restore, maintain, improve or replace tissues or whole organs. The main goal tissue engineering is to overcome the restrictions of existing treatments that are based on organ transplantation. Cells and biomaterials can be used to form functional tissues and organs. Actually, the goal is to produce structures that resemble and mimic the real tissues. One of the useful mimicking technique is cell patterning. Cell patterning is a technique that provides cell clusters are located at a proper position for function of tissues. Some of the patterning techniques uses cell adhesion ligands, optical tweezers, acoustic tweezers, dielectrophoresis and magnetic force. In addition to the advantages of all these techniques, there are also disadvantages. However, Magnetic force-based cell patterning techniques provide excellent advantages such as low adverse effects to cell. Magnetophoresis is one of the magnetic force-based cell patterning technique that forms cell patterns without labeling cells in a short time using the principle of movement of the cells to lower magnetic field region in a paramagnetic medium. In this study, a cell patterning system was used to form cell patterns via magnetophoresis. Results showed that cell patterns were formed in different shapes in a short time and they maintained integrity even if magnetic force was removed.