Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Author: search.filters.author.Akdoğan, YaşarSubject: search.filters.subject.Drug delivery systems

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  • Master Thesis
    Preparation of Drug Loaded Albumin Nanoparticles in Water / Ionic Liquids Microemulsion Systems
    (Izmir Institute of Technology, 2021) Yıldırım, Barış; Akdoğan, Yaşar
    Nanoparticles (NPs) have been used in various applications such as biotechnology, nanomedicine, and drug delivery systems. Many nanoparticle drug delivery systems have been promoted for cancer treatment, and numerous materials have been investigated to use as drug delivery agents to enhance the therapeutic efficiency and safety of anticancer drugs. Albumin is a natural biopolymer and the most abundant protein in blood plasma. Due to its versatile binding capacity of widespread therapeutical drugs, albumin becomes an ideal material to obtain nanoparticles. In this study, the ionic liquid (IL) based emulsification methods were investigated. Instead of classical toxic and volatile solvents, using ILs in microemulsions, environment-friendly media were received to synthesize bovine serum albumin (BSA) NPs. In order to obtain BSA NPs, high-speed homogenizer processing was applied by following crosslinker addition. The IL microemulsions are a thermodynamically stable colloidal dispersion containing spherical droplets (W/IL or IL/W) in submicron sizes that act as nanoreactors for NP formation. Chlorambucil (CHL) was used as a model drug to investigate drug loading and releasing kinetics of BSA NPs as a drug delivery candidate. Results showed that chlorambucil loading capacities and release kinetics depended on the synthesized medium such as anion-type of ILs and surfactants. CHL loaded to the BSA NPs synthesized in hydrophilic IL BmimBF4 in relatively higher amounts and released in the same trend. In addition, the cell viability effect of CHL-loaded BSA NPs synthesized in different types of ILs were investigated. The CHL-loaded BSA NPs synthesized in BmimOTf and BmimPF6 reduced the cancer cell viability more than the used same dose of free CHL.
  • Master Thesis
    Study of Drug Transportation by Esr Spectroscopy
    (Izmir Institute of Technology, 2018) Tatlıdil, Duygu; Akdoğan, Yaşar; Emrullahoğlu, Mustafa
    The ability to track drug binding and release makes electron spin resonance (ESR) spectroscopy well suited for drug delivery studies. Using the continuous wave cw ESR technique to extract information about the dynamics of the spin labeled drugs we can simultaneously determine the bound and unbound drugs. In this study, ESR technique was used to detect the binding and release of spinlabeled salicylic acid (SLSA) to and from bovine serum albumin (BSA), and to detect different binding interactions between them. We have labeled salicylic acid with stable nitroxide-based tempo radicals to monitor the BSA bound and unbound conditions of the drug. Studying with the different concentrations of SLSA-BSA binding showed that the drug-protein stoichiometry increases significantly in the physiological range of BSA concentration. Also, during the release of SLSA from BSA, there is an unchanging balance between the bound and unbound SLSA. In order to study various drug binding interactions, SL-benzoic acid, SL-phenol, SL-benzene, SL-cyclohexane, SL-hexane and SL-methyl were prepared. We showed that the main conjugation in the binding of these drugs to BSA is hydrophobic interaction. In addition, cationic BSA (cBSA) was prepared to investigate the effect of electrostatic interaction on drug binding. The SLSA loading capacity of cBSA is significantly higher than that of BSA, this result indicates the importance of electrostatic interactions for the drug binding. Finally, we examined the competitive binding behaviors of salicylic acid, ibuprofen and aspirin to BSA. Binding sites of SL-salicylic acid and SL-ibuprofen in BSA show 96% of similarities. In addition, our results showed that binding sites of SL-salicylic acid and SL-aspirin in BSA have 73% of similarities.These results demonstrate that cw ESR spectroscopy with the spin labeling technique is an effective technique for the determination of drug-protein interactions and stoichiometric analysis of drug binding.