Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Encapsulation of Sarcopoterim Spinosum Extract in Zein Perticle by Using Electrospray Method(Izmir Institute of Technology, 2013) Süngüç, Ceren; Bayraktar, OğuzSarcopoterium spinosum species has valuable and common medicinal plant in the Mediterranean region. The optimum conditions for the extraction of S. spinosum leaves to obtain bioactive extract were investigated using response surface methodology (RSM). Total phenol contents, total antioxidant and antibacterial activities, phenolic composition of S. spinosum extract were studied. The prepared S. spinosum extract showed high antioxidant activity when compared with many other medicinal plants in the literature. It was determined as 3143.5 mmole Trolox per gr dry weight. The phenolic content of S. spinosum extract was examined with High Performance Liquid Chromatography (HPLC). Hyperoside and isoquercetin were detected in S. spinosum extract. Especially, isoquercetin was the major compound in the extract. In addition, the antimicrobial activity of S. spinosum extract was investigated. The extract showed fungicide activity against Candida albicans. S. spinosum extract were encapsulated within zein particle via electrospray method in order to enhance its stability. The effects of process parameters for electrospraying method on the particle morphology and size distribution were extensively investigated. The best process conditions were determined as zein concentration of 5% (w/v) in 70% aqueous ethanol solution, flow rate of 0.3 ml/h and applied voltage of 14 kV depending on narrow size distribution, spherical and smooth particle morphology. The best S. spinosum extract loading was achieved at extract to zein weight ratio of 1:5. The prepared extract loaded zein microparticles showed significant antioxidant activity.Master Thesis Screening of Some Plant Species for Their Total Antioxidant and Antimicrobial Activities(Izmir Institute of Technology, 2008) Kaçar, Diren; Bayraktar, OğuzIn this study aqueous/ethanol extracts of 42 plant species collected from same geographic region (Karaburun/İzmir) were screened for their relative total phenol contents, total antioxidant and antibacterial activities. In the first part of the study, Folin- ciocalteu assay and PCL (Photochemiluminescence) method were performed to detect the total phenol contents and total antioxidant activities of extracts, respectively.It was detected that the Hypericum empetrifolium had the highest activities for both water soluble and lipid soluble antioxidants and Sarcopterium spinosum has the highest result for total phenol assay as 635.26 GAEqmg/g sample.In order to detect the antibacterial activities of extracts a preliminary screening study was performed by using disc diffusion method. Out of the 42 plant species tested, 26 species exhibited antibacterial activities by inhibiting one or more microorganisms.Microdilution assays by 96 well plates were applied for the most active species to find out their minimum inhibition concentrations (MICs). The most promising plant species in the study, having the antibacterial activities were determined as H. empetrifolium, P.terebinthus, Arbutus unedo, and C. parviflorus.In this study there is a clear relationship between the analysis results and S.spinosum is one of the most noteworthy species in this study showing the highest total phenol content and important biological activities which has never been examined scientifically before. In that manner this study also presents new potential species that can be used as natural raw materials in some related industries.