Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Effect of Edible Film and Allicin on Shelf Life of Chicken Meat(01. Izmir Institute of Technology, 2024) Baysal, Ayşe Handan; Baysal, Ayşe Handan; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyTavuk eti besleyici değeri sebebiyle sağlıklı beslenme için önemli bir gıdadır ve büyüme çağındaki çocuklar ve yaşlılar gibi toplumun çoğu popülasyonu tarafından tüketilen bir gıdadır. Ancak tavuk eti kolay bozulabilen bir gıdadır ve bunun önüne geçmek için yenilebilir kaplamalardan ve biyoaktif bileşenlerden yararlanılmaktadır. Bu çalışmada, kitosanın antibakteriyel, antimikrobiyal, biyouyumlu ve biyolojik olarak parçalanabilir özellikleri ve allisinin antimikrobiyal, antifungal ve antioksidan özellikleri kullanılarak tavuk bagetlerdeki raf ömrüne etkisi incelenmiştir. Tavuk bagetlerin, farklı konsantrasyonlarda hazırlanan kitosan ve allisin çözeltileriyle K1A1(1% Kitosan + 10% Allisin), K1A2 (1% Kitosan + 20% Allisin), K2A1(2% Kitosan + 10% Allisin), K2A2(2% Kitosan + 20% Allisin) ve sadece kitosanın farklı konsantrasyonlarını içeren çözeltileriyle (%1 Kitosan, %2 Kitosan) kaplanması, mikrobiyolojik özellikleri, duyusal özellikleri, kimyasal parametleri, oksidatif bozulma parametreleri belirlenen raf ömrü boyunca 4 ⸰C'de saklanarak değerlendirilmiştir. Kitosan ve allisin ile kaplanan numunelerde ve kitosan çözeltisiyle kaplanmış numunelerde kontrol grubuna kıyasla toplam canlı, Enterobacteriaceae, Pseudomonas bakteri sayımlarında, bakteri sayısı önemli ölçüde azalmıştır. Toplam canlı gelişimini engellemede en etkili sonuç, tavuk bagetlerin raf ömrünü uzatan K2A1 örnek grubuyla elde edildi. Depolamanın 10. günündeki TBA sonuçlarında, %1 kitosanla kaplanmış tavuk bagetlerin 0.33 mg MDA/kg değeriyle lipid oksidasyonunu engellemede en başarılı grup olduğu bulunmuştur. Bu sonuçtan sonra en başarılı sonuç 0.55 mg MDA/kg değeriyle K1A2 örneği olmuştur. Anahtar Kelimeler: Allisin, Antimikrobiyal,Antioksidan, Kitosan, TBA, Toplam canlıMaster Thesis Investigation of Antimicrobial, Antioxidant and Cytotoxic Properties of Some Green Leaf Plants(2023) Baysal, Ayşe Handan; Özen, Fatma Banu; Baysal, Ayşe Handan; Özen, Fatma Banu; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe present study aims to investigate the chemical composition, antimicrobial activity, antioxidant properties, and cytotoxic activity of extracts obtained from leaf samples that are discarded as waste products. For this purpose, two different green leaves were selected: one is the grape (Vitis vinifera) leaf, a by-product from the winemaking process, and the other is the cauliflower (Brassica oleracea, var. botrytis) leaf, which is a waste product of cauliflower, and they were subjected to related tests. Both leaf samples were extracted by water because of its easy accessibility and environmentally friendly properties. Leaf extracts were examined according to their total phenolic content and subjected to chemical characterization by Liquid chromatography-quadrupole time-of-flight tandem mass spectrometry system. The promising antioxidant activities of the water-extracted leaf samples were authenticated through DPPH and ABTS assays. Antimicrobial and antibiofilm activities were examined against some Gram-positive (Bacillus cereus, Listeria innocua, and Carnobacterium divergens) and Gram-negative (Escherichia coli, Serratia liquefaciens, and Salmonella Typhimurium) strains, and two fungi (Saccharomyces cerevisiae and Candida albicans) species. Both tested leaf extracts showed a dose-dependent antimicrobial activity, while the antimicrobial activity of grape leaf extract was slightly higher. However, their activities against biofilm formation were varying in different bacterial and fungi species. The cytotoxic activity of the leaf extracts was examined on the mouse fibroblast cell (L929) line. According to the presented results, neither of the leaf extract samples used in the study showed any unwholesome effects on the cell line at any time point.Master Thesis Antimicrobial, Antioxidant, Antiproliferative and Cytotoxic Activities of Aronia Fruit Extract(01. Izmir Institute of Technology, 2023) Baysal, Ayşe Handan; Sezgin, Efe; Baysal, Ayşe Handan; Sezgin, Efe; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyAronia melanocarpa contains high amounts of phenolic compounds, especially anthocyanins. Because of its high chemical content and significant antioxidant action, this food is known as a functional food, and its use is spreading worldwide. Within the scope of this thesis research, studies were carried out on Aronia melanocarpa dry extract and liquid extract. For these purposes, chromatographic and chemical profile were determined in detail by HPLC (High-Pressure Liquid Chromatography), Fourier-Transform Infrared Spectroscopy (FTIR) and Q-TOFF- MS (Quadrupole Time of Flight Mass Spectrometry), and significant bioactive were determined. Spectroscopic methods were used to characterize phenolic, anthocyanin, and flavonoid components. Aronia melanocarpa dry and liquid extracts were tested for antioxidant activity using DPPH and ABTS methods. The antioxidant potential of the Aronia melanocarpa dry and liquid extracts studied is high. To test the antiproliferative and cytotoxic effects, cytotoxic studies were performed on the CaCo2 cell line. Cell migration was also studied in HUVEC and HaCat cell lines. The antimicrobial activity of Aronia melanocarpa dry extract and liquid extract was tested against Saccharomyces cerevisiae, a yeast species, and bacterial strains of Staphylococcus aureus, Escherichia coli O157:H7, Salmonella Typhimurium. The extracts analyzed showed an antimicrobial effect on the tested bacteria at different concentrations. The results obtained in this study emphasize that the tested Aronia melanocarpa dry extract and liquid extract have antimicrobial properties. Chromatographic, chemical, and cytological data reveal that Aronia melanocarpa liquid and dry extracts can be used as antioxidative and antiproliferative products as food supplements in the health field.