Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

Browse

Filters

2017 - 201912020 - 20233

Settings

Author: search.filters.author.Bulmuş Zareie, Esma VolgaSubject: search.filters.subject.Biosensors

Search Results

Now showing 1 - 4 of 4
  • Master Thesis
    Effect of Gold Nanorod Properties on Lspr Response
    (01. Izmir Institute of Technology, 2023) Bulmuş Zareie, Volga; Tekin, Hüseyin Cumhur; Bulmuş Zareie, Esma Volga; Tekin, Hüseyin Cumhur; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Optical qualities make gold nanorods (GNRs) excellent for plasmonic biosensors. Localized surface plasmon resonance (LSPR) phenomenon which occurs on GNR surfaces enables the creation of highly sensitive biosensors. The physical properties such as aspect ratio and size are directly related to the LSPR response of GNRs. The aim of this study is to investigate the impact of the aspect ratio (AR) and the interparticle distance on the localized surface plasmon resonance (LSPR) response of GNRs decorated glass sensor chips. For this aim, GNRs were first synthesized using a seed-mediated growth method. The effect of AgNO3 concentration on the AR of GNRs was investigated. It was observed that increasing AgNO3 concentration resulted in GNRs with higher AR and a red shift in the longitudinal plasmon peak wavelength. GNRs with an AR of 4, 6 and 8 were successfully synthesized. Next, the effect of the stabilizer molecule type and molecular weight on the distribution of GNRs on the silanized glass surface was investigated. It was found that the APTES modified glass surfaces cannot be coated with CTAB stabilized GNRs. Using GNRs stabilized with PEG5K resulted in a more homogeneous distribution of GNRs on the glass surface with respect to GNRs stabilized with PEG2K. The interparticle distance between GNRs on the glass surface was successfully controlled by simply concentrating or diluting the GNR solution used for coating the glass surfaces. It was observed that the LSPR peak shifts decreased upon binding of analytes as the interparticle distance between GNRs decreased in the studied range. On the other hand, as the AR decreased, the LSPR response of the GNRs shifted blue. The results presented in this thesis may contribute to future research to improve the potential of LSPR-based biosensors for diverse biomedical and diagnostic applications.
  • Master Thesis
    Development of an Advanced Lspr-Based Biosensor Chip for Rapid Detection of Border Disease Virus
    (01. Izmir Institute of Technology, 2023) Bulmuş Zareie, Volga; Tekin, Hüseyin Cumhur; Bulmuş Zareie, Esma Volga; Tekin, Hüseyin Cumhur; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    The Border Disease Virus (BDV) is responsible for causing fetal deathly infection, leading to annual occurrences of affected farms. BDV, along with other pestiviruses such as classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV), are known to cause major losses in stock farming. These losses can result in reproductive failure, expensive inspections, and other impacts on livestock health. The current detection methods of BDV include various techniques such as RT-PCR, ELISA, VNT, and immunofluorescence assays. These methods, although reliable, may require specialized equipment, time-consuming procedures, and laboratory facilities, making them less suitable for rapid on-site detection. Hence, it is imperative to employ diverse methodologies for detection of BDV. LSPR-based biosensors are a subset of plasmonic biosensors that exhibit numerous advantages for diverse applications. LSPR-based biosensors are particularly well-suited for the production of compact, practical devices for rapid, on-site detection of analytes. The aim of this study is to design and fabricate a biosensor chip utilizing LSPR technology for potential BDV detection. For this aim, glass surfaces were functionalized with gold nanorods modified with a BDV-specific primer sequence, complementary single-strand DNA sequence of 19 bases, and fabricated with PMMA microchannels. Different concentrations of target BDV-DNAsequence ranging from 0.01 pM to 100 nM were exposed to the channels, and the LSPR response was quantified using a Vis-NIR spectrometer. The limit of quantification of the biosensor chips was determined to be 10 pM, while the limit of detection was found to be less than or equal to 1 pM. The sensitivity of the biosensor chips was calculated to be 0.0567 nm/RIU. The dynamic range of the biochips lies between 10 pM to 100 pM.
  • Master Thesis
    Design of Localized Surface Plasmon Resonance (lspr) Based Biosensor for Detecting a Potential Cancer Biomarker
    (Izmir Institute of Technology, 2020) Bulmuş Zareie, Volga; Bulmuş Zareie, Esma Volga; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Conventional methods for detection of cancer are invasive, expensive and not suitable for early diagnosis. Therefore, demand for simple, sensitive and rapid biosensors for detection of cancer have been enormous. Gold nanorods (GNRs) have been ideal materials for utilization in biosensors because of their exceptional optical properties. Localized surface plasmon resonance (LSPR) which is created on GNR surface can be used for the development of label-free and sensitive biosensor systems. LSPR responds to changes in the refractive index of the surroundings and this change can be observed as the shift in the maximum absorption wavelengths. In this thesis, an LSPR based GNR biosensor was developed for sensitive detection of a sialic acid as a potential cancer biomarker. For this purpose, GNRs were synthesized at around 40-50 nm in length. Afterwards, glass surfaces were coated with GNRs and functionalized with self-assembling molecules. Specific monoclonal antibodies(Ab) were conjugated to the surface. The surface modifications were characterized via contact angle, scanning electron microscope, Fourier transform infrared spectroscopy and zeta potential. Ab-functionalized glass surfaces were used to quantitatively detect specific molecular bindings via LSPR. The sensitivity of the biosensor was determined as 281 RIU/nm. The detection limit in PBS was 1 nM, while in serum it was found to be as 10 nM because of the high protein content of serum. Control experiments showed that the developed biosensor chip was selective. The proposed system is promising for early diagnosis of cancer since it can detect a potential cancer biomarker at concentrations as low as nanomolar level.
  • Master Thesis
    Design and Construction of Portable Localized Surface Plasmon Resonance Device for Detection of Biological Molecules
    (Izmir Institute of Technology, 2017) Gül, Aytaç; Bulmuş Zareie, Volga; Bulmuş Zareie, Esma Volga; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Point-of-care devices giving rapid results in non-laboratory settings have become important for biosensor applicattions in a wide range of fields including medical, food, agriculture and pharmaceutical. This work aims to portable device based on localized surface plasmon resonance spectroscopy (LSPR-S) as a potential biosensor platform that can be used in non-laboratory settings for rapid detection of biological molecules at hifh sensivity. The thesis can be divided into two parts: In the first part, the design and consctruction of the device including both the mechanical and electronic parts are presented. The mechanical section includes the integration of the parts to build the device and microchannels designed with the aid of a three-dimensional drawing program Solid Works 2015. The second step of the construction process was the installation of electronic components onto the device. The electronicpart consists of a light source, fiber optic cables, a spectrometer and a temperature sensor. In conclusion, a portable LSPR-S device with an integrated microchannel system has been produced, which potentially allows analysing low volumes of sample without the need to label the molecules. The second part of the thesis covers the studies towards the preparation and application of sensing platforms for the LSPR-S device constructed to enable the rapid detection of biplogical molecules at high sensitivity. These included the preparation of gold nanorods and nanoparticles-based LSPR-S detection of model antibody-antigen and bacteria-bacteriophage interactions, respectively. Studies conducted in this section have led to the conclusion that LSPR-S-based biosensor platforms developed in thisis are promising solutions to overcome current challenges in biosensor applications.