Master Degree / Yüksek Lisans Tezleri

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Author: search.filters.author.Bulut Albayrak, ÇisemSubject: search.filters.subject.Probiotics

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  • Master Thesis
    Modulating Akkermansia Muciniphila by Probiotics for Obesity Management
    (Izmir Institute of Technology, 2021) Kamber, Aslıhan; Harsa, Hayriye Şebnem; Bulut Albayrak, Çisem
    The aim of this thesis is to investigate the potential of lactic acid bacteria (LAB) having probiotic properties to trigger Akkermansia muciniphila as anti-obesity candidate. Artisanal strains of LAB were evaluated in terms of stability in gastrointestinal conditions, mucin/mucus non-utilization properties, growth in the presence of mucin, adhesion to the mucin/mucus layer, biofilm formation, lactate production abilities, antimicrobial properties, HT-29 cell binding and mucin/mucus thickness increment abilities. Among 40 strains, 17 of LAB including 6 olive isolates were selected for their tolerance to gastrointestinal conditions and analyzed for further abilities e.g., mucin promotion potential. Most of them (13 of the 17 bacteria) remained viable in the range of 106-108CFU/mL in the simulated gastric juice, and these bacteria continued their viability in the range of 105-107 CFU/mL in the simulated intestinal fluid. Besides, 6 reference and 7 L. bulgaricus isolates did not utilize the mucin and showed no or negligible growth in the presence of mucin. Additionally, 13 of the 17 bacteria adhered to mucin layer with variable degree of binding between 41.03-65.37%. Furthermore, variable degree biofilm formation efficiencies can also support their existence and adhesion to intestinal cells. Varying amounts of lactate by 7 LAB were produced (6.51-18.17 g/L) and this ability is another important criteria for supporting mucus pathway. All those strains adhered to HT-29 cell line and they showed significant increase in mucin thickness by comparing the control. L. plantarum C47 and Lactobacillus AK50 isolates were selected as the antiobesity prototype candidates for their desirable attributes.