Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Induction of Hairy Root Culture in Eggplant With Rhizobium Rhizogenes(Izmir Institute of Technology, 2021) Öztürk, Tuba; Doğanlar, Sami; Öztürk, Tuba; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 01. Izmir Institute of Technology; 04. Faculty of ScienceIn this study, It has been used two agropine strains (ATCC 15834 and ATCC 43057) of R. rhizogenes to compare hairy root induction in hypocotyl and cotyledon explants of eggplant. Transformation frequency was found to be the highest in hypocotyl explants (49.4%) infected with ATCC 15834. Thirty nine hairy root clones were selected based on their hairy root phenotype. The transgenic status of 15 of these clones was tested by PCR using rol gene primers. Hairy roots infected with ATCC 15834 were grown for 8 weeks in MS liquid medium. At the end of this growth period, the growth ratio of the hairy roots was measured. The root biomass was 6.52 g/DW for hypocotyl and 9.83 g/DW for cotyledon-derived clones. Biochemical analyses of clones were performed. Total phenolic contents were evaluated for hypocotyl and cotyledon-derived clones yielding 8.56 mg/g DW and 8.81 mg/g DW, respectively. The average total flavonoid content was 2.12 mg/g DW in hypocotyl-derived clones, and 1.90 mg/g DW in cotyledon-derived clones. On average, these amounts of both phenolic and flavonoid contents were significantly higher than those obtained for untransformed eggplant roots. Overall the results indicated that eggplant hairy roots are a useful source of secondary metabolites. In the future, the production of metabolites of interest such as steroidal glycoalkaloids or phenolics can be improved using elicitors in eggplant.Master Thesis Genetic Mapping and Characterization of Eggplant for Glycoalkaloid Content(Izmir Institute of Technology, 2010) Gürbüz, Nergiz; Gürbüz, Nergiz; Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyMost plants including eggplant, produce toxins against insects, pathogens and animals. The biggest class of these toxins is alkaloids. Generally plants produce alkaloids in a glycosidic form which are called glycoalkaloids. Glycoalkaloids have toxic effects on human health. For example, as a result of disrupting the cell membrane, the gastrointestinal track and other organs are damaged. Glycoalkaloids are not only toxic to human health but also they have beneficial effects. For example, they decrease cholesterol level, and have anticancer activity. And also they are used as a precursor for steroidal drugs. Therefore, isolation and determination of glycoalkaloids content is important. For this aim, column chromatography was performed and solamargine and solasonine were obtained. Totally 122,8 mg solamargine, solasonine and solamargine and solasonine mix from Solanum linnaeanum were obtained. Their identities were confirmed via 1H NMR and mass spectroscopy. For determination of glycoalkaloid content HPLC method was developed via changing different parameters such as mobile phase, pH, temperature, flow rate and column type. It was found that separation of glycoalkaloids by using RP column without phosphate buffer was not sufficient. On the other hand, separation of glycoalkaloids by using carbohydrate column was better but this type of column is not stable. Furthermore, linkage mapping was performed by using CAPs and SSR methods; and 385 COSII markers and 221 SSR markers were tested. Of the tested markers, 38 of the COS II markers and 28 of the SSR markers were found to be polymorphic. A total of 52 of these markers were mapped.