Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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2007 - 200912010 - 20111

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Author: search.filters.author.Doğanlar, SamiSubject: search.filters.subject.Plant genetic engineering

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  • Master Thesis
    Development of Ssr Markers in Poppy (papaver Somniferum L.)
    (Izmir Institute of Technology, 2011) Çelik, İbrahim; Doğanlar, Sami
    The opium poppy (Papaver somniferum L.) belongs to the family Papaveraceae. Opium poppy is an important agronomic plant due to its production of more than 80 different alkaloids. In addition, poppy oil and seeds are used in food. The number of molecular markers for opium poppy is limited. To date, molecular characterization of opium poppy has been done using general marker systems such as AFLP, RAPD, ESTSSR and ISSR. However, development of other molecular markers systems is essential for more comprehensive analysis of the opium poppy genome. A genomic library was constructed from opium poppy DNA. Genomic DNA was sequenced by Roche 454 SequencingTM platform. Genomics reads were clustered by MIRA software. Di-, tri-, tetra-, penta- and hexanucleotide SSR repeats were identified and flanking primers were designed by Batchprimer3 software. A total of 1399 contigs containing 3284 SSRs were identified. A total of 1820 primer pairs which fulfilled the criteria for primer design were designed from flanking sequence of SSRs. A total of 100 SSR primers were tested in six Papaver somniferum accessions. No polymorphism was found among the Papaver somniferum accessions. Transferability of the markers was tested in seven Papaver species. SpSSR-6, spSSR-8 and spSSR-23 detected polymorphic alleles in these species. These markers are the first set of opium poppy-specific SSR markers derived from genomic sequence of this crop. These markers can be used for assessment of genetic diversity, mapping and marker assisted selection in opium poppy.
  • Master Thesis
    Determination of Genetic Diversity Between Eggplant and Its Wild Relatives
    (Izmir Institute of Technology, 2007) Tümbilen, Yeliz; Doğanlar, Sami
    Eggplant (Solanum melongena L.) is an important crop and has a growing reputation and is now cultivated globally. It is a valuable member of the human diet in Asia, especially in India, which is a primary diversity center of the species. Turkey is the first in Europe and is in the first five countries around the world in terms of eggplant production. The Solanaceae family to which S. melongena belongs is an important family, too. Tomato, potato, tobacco and petunia are some example species of the Solanaceae family. This important family with 3000-4000 species shows a high level of morphological diversity which results in confusion about its systematics and this diversity is at the level of genera, species and cultivars. The aims of the studies reported in this thesis were to analyze genetic diversity of Turkish eggplants and wild relatives in separate studies with different molecular tools. To reveal genetic diversity among eggplant cultivars grown in Turkey, the AFLP marker system was applied to the sample genotypes. For the investigation of genetic variation between S. melongena and its wild relatives, though, the SSR marker system was used. For the AFLP data for Turkish eggplants, an r value of 0.97 was obtained which was in the best scale. Eigen values reported here were also informative. These results showed that the first component analysis explained 64.34% of the variation between samples. For three axes, though, a total of 72.21% variation was explained. According to the statistical results of SSR analysis, the r value of Solanum species. genotypic data was found to be 0.88. That means the correlation between sample genotypic data and dendrogram was found to be high. Due to the other statistical results which were Eigen values explained 46.12% of genotypes for first component analysis. With a total value of 55.28%, the 47 different genotypes were explained by the three principle component axes. The results of AFLP studies showed that although a high similarity value was observed, diversity was detectable among the accessions. The results of SSR studies were also meaningful with their concordance with previous studies and observed diversity with a good fit to statistical results.