Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Development of Molecular Markers Linked To Me1 Gene Conferring Resistance To Nematode in Pepper(Izmir Institute of Technology, 2012) Öztürk, Leyla; Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyPepper (Capsicum annuum L.) belongs to family Solanaceae. It is an agronomically important plant originating from Mexico. Pepper yield, quality and growth are limited by plant nematode parasitism. Root-knot nematodes (Meloidogyne spp.) are the most damaging sedentary endoparasites. M. incognita race 2 is the most common root-knot nematode found in Turkey. The Me1 gene which confers resistance to M. incognita was mapped in pepper to a 28 cM interval on chromosome 9. The aim of this study was to develop molecular markers linked to this nematode resistance gene. Phenotypic characterization of nematode resistance was performed for 200 F2 individuals from the cross between resistant pepper cultivar PM217 and Turkish susceptible cultivar AZN-1. In the F2 individuals, 151 plants (76%) were evaluated as resistant, 49 plants (24%) were susceptible. Markers were tested on parents of the mapping population to identify polymorphisms. A total of 30 standard markers and 548 new marker combinations were tested. Of these 578 markers, 75 (13%) were polymorphic. They were applied to F2 population and 28 (37%) showed clear segregation on F2 population. Eighteen of the markers (64%) segregated dominantly, 10 of the markers (36%) segregated codominantly. Markers located near Me1 on chromosome 9 were used for the construction of a linkage map. Out of three markers, SCAR_CD was the nearest marker to Me1 gene with a distance of 1.1 cM. These markers will provide selection at the genotypic level by marker-assisted selection, which will increase the efficiency and effectiveness of pepper breeding for nematode resistance.Master Thesis Determination of Genetic Diversity of Turkish Sesamum (sesamum Indicum L.) by Using Aflp Markers(Izmir Institute of Technology, 2011) Tekin, Pelin; Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologySesame (Sesamum indicum L.) belongs to the Pedaliaceae family. It is an important oil seed crop which is cultivated in tropical and subtropical areas of Asia and Africa. China is the largest producer of sesame seed in the world while Turkey ranks seventh and produces 21036 tonnes of sesame seed in a year. Although sesame's edible seed and high quality seed oil are important for both humans and the economy, there is not enough information about the sesame genome in the literature. Our aim was to determine the diversity of 158 Turkish sesame accessions by using the AFLP marker system and to design a new set of sesame-specific SSR markers from genomic sequence of S. indicum. The Turkish sesame accessions were tested with five AFLP primer combinations, as a result, 148 polymorphic fragments were obtained. The maximum similarity was 57% for the accessions and a good level of diversity was present in the sesame germplasm. Secondly, a genomic library of sesame was constructed. A total of 1.094.317 reads were obtained and 702.371 of them were clustered to 140.669 reads containing 93.365 nucleotides. A total of 3101 primer pairs were developed from flanking regions of SSRs with primers for dinucleotide (36,4%), tetranucleotide (29,3%), trinucleotide (23,1%), pentanucleotide (7,1%), and hexanucleotide (4,2%) repeats. These primers are the first genomic-SSR markers developed for sesame cultivars. SSRs have good reproducibility, high genome coverage, co-dominant inheritance, good transferability to close species and are multiallelic. The designed genomic-SSRs should be very useful for sesame mapping and diversity studies.