Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Author: search.filters.author.Doğanlar, SamiSubject: search.filters.subject.Plant molecular genetics

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Now showing 1 - 4 of 4
  • Master Thesis
    Induction of Hairy Root Culture in Eggplant With Rhizobium Rhizogenes
    (Izmir Institute of Technology, 2021) Öztürk, Tuba; Doğanlar, Sami
    In this study, It has been used two agropine strains (ATCC 15834 and ATCC 43057) of R. rhizogenes to compare hairy root induction in hypocotyl and cotyledon explants of eggplant. Transformation frequency was found to be the highest in hypocotyl explants (49.4%) infected with ATCC 15834. Thirty nine hairy root clones were selected based on their hairy root phenotype. The transgenic status of 15 of these clones was tested by PCR using rol gene primers. Hairy roots infected with ATCC 15834 were grown for 8 weeks in MS liquid medium. At the end of this growth period, the growth ratio of the hairy roots was measured. The root biomass was 6.52 g/DW for hypocotyl and 9.83 g/DW for cotyledon-derived clones. Biochemical analyses of clones were performed. Total phenolic contents were evaluated for hypocotyl and cotyledon-derived clones yielding 8.56 mg/g DW and 8.81 mg/g DW, respectively. The average total flavonoid content was 2.12 mg/g DW in hypocotyl-derived clones, and 1.90 mg/g DW in cotyledon-derived clones. On average, these amounts of both phenolic and flavonoid contents were significantly higher than those obtained for untransformed eggplant roots. Overall the results indicated that eggplant hairy roots are a useful source of secondary metabolites. In the future, the production of metabolites of interest such as steroidal glycoalkaloids or phenolics can be improved using elicitors in eggplant.
  • Master Thesis
    Molecular Genetic Analysis in Sesame (sesamum Indicum L.)
    (Izmir Institute of Technology, 2010) Seyitalioğlu, Ümmü; Doğanlar, Sami
    In this study, 156 Turkish sesame (Sesamum indicum L.) accessions were characterized morphologically and 161 accessions were characterized genotypically. A total of 15 criteria were used for morphological characterization. Simple Sequence Repeats (SSRs, microsatellites) were used to characterize genetic variability among Turkish accessions. A total of 318 new EST based SSRs were developed for genotypic analysis. Also, these microsatellites were screened in Korean-Japan and African sesame accessions (parents) for future map construction studies. According to the morphological analysis, some characters showed variation whereas some characters had no variation among accessions. For example, height of first capsule, capsule number per plant, plant height, number of branches, number seeds/capsule, days to 1st flower and days to %50 flower characters showed variation among accessions. However, stem hair, leaf hairs, axil flower number, number of carpels/capsule and capsule hairs showed variation for only a few accessions. Also, growth habit, branching and capsule splitting characters had no variation. In accordance with genetic characterization, survey results showed only five polymorphic markers among 318 EST based SSR markers. Also, in this study, genetic distance of Turkish sesame accessions was calculated using DICE matrix and UPGMA (Unweighted Pair Group Method) arithmetical averages via 19 EST based SSR fragments. Genetic analysis showed that Turkish sesame accessions have fairly low genetic diversity. As a result, genetic diversity among Turkish sesame accessions were defined and the genetic relationships of Turkish sesame accessions were established.
  • Master Thesis
    Molecular Mapping of N Gene Conferring Resistance To Root-Knot Nematodes in Pepper
    (Izmir Institute of Technology, 2013) Arslan, Mehmet Enes; Doğanlar, Sami; Doğanlar, Sami
    Pepper (C. annuum) is one of the most important agricultural crops worldwide and Turkey ranks third among all countries in pepper production. Pepper species have economical and also pharmaceutical importance so, it is vital to develop different methods to increase pepper yields. The root-knot nematode (Meloidogyne species) is one of the most important biotic factors that affect pepper growth and development in Turkey. The dominantly inherited N gene which was mapped on chromosome P9, 7 cM from Me1 and 2 cM from Me3, confers resistance to pepper species against Meloidogyne species. The aim of this work was to develop a marker tightly linked to the N gene which can be used in marker-assisted selection. A total of 132 SSR Hpms primers, 230 EST-SSR markers and 45 chromosome 9 specific primers were used to a construct linkage map and find an N linked marker. Hpms SSR markers gave 19% polymorphism by capillary electrophoresis, EST-SSR markers showed 5.2% polymorphism by agarose gel electrophoresis while the chromosome 9 specific markers, yielded 20% polymorphism by fragment analyzer. When all 407 analyzed markers are considered, only 11.3% polymorphism was observed and these results were expected because we used an intraspecific population. The, polymorphic markers were mapped in a "Carolina Wonder" X "AZN-1" F2 population and analyzed with JoinMap software. Three markers were linked with the N gene. These markers are ScarPM6a (3.6 cM), ScarPM6b (10.2 cM) and ScarN (22.6 cM) which are located with same segregation group with the N gene. These markers will allow development of a marker tightly linked to the N gene which can be used in marker-assisted selection to increase the efficiency and effectiveness of pepper breeding for nematode resistance.
  • Master Thesis
    Genetic Diversity of Turkish Spinach Cultivars
    (Izmir Institute of Technology, 2011) Avşar, Bihter; Doğanlar, Sami
    In this study, 95 Turkish spinach (Spinacia oleracea L.) accessions were used for genetic diversity analysis by using the SRAP (Sequence Related Amplified Polymorphism) marker system and also 81 of the spinach accessions were used for morphological data analysis using data from the Centre for Genetic Resources, Wageningen University, The Netherlands. In the study, a total of 25 SRAP marker combinations were used and 19 of these were suitable for genetic analysis because they gave amplification and they were polymorphic. For the 19 SRAP markers, 123 bands were amplified and 67 were polymorphic. These polymorphic bands were used to construct a dendogram for spinach cultivars and to determine the genetic distance. Dendrogram analysis was done UPGMA method which was calculated using DICE matrix. Genetic similarity ranged between 0.30 and 0.95. Group B had most related accessions, 0.77 similar, and Group A, C and D had more distinct accessions, 0.60 similarity. For the morphological data PCA(Principal Component Analysis) was performed and no any apparent group was seen so high diversity was indicated by the 2D plot. All these results showed that the SRAP marker system was a suitable marker to determine genetic diversity, that Turkish spinach germplasm is diverse and that accessions should be preserved. Another importance of this results is that it is the first and only study that has been done with Turkish spinach germplasm.