Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Physiological and Biochemical Characterization of Drought Tolerance in Chickpea(Izmir Institute of Technology, 2012) Keskin, Hilal; Frary, AnneChickpea (Cicer arietinum cv. Gokce.) is an agronomically and economically significant plant for Turkey. It is successfully grown under severe drought conditions which limit the growth of other plants. It is generally affected by terminal drought which causes retardation of flowering and decreases yield in Mediterranean and subtropical climates. The aim of this study was to determine significant factors which can be used to identify chickpea plant tolerance to drought stress. With this objective we assessed physiological (fresh and dry weight, relative and real water content) and biochemical (enzymatic and non-enzymatic antioxidants, malondialdehyde, total protein and phytohormone contents) parameters which were used to measure the impact of drought on chickpea. To determine drought's effects, we collected stressed (drought treated) and control (non drought treated) samples from the chickpea cultivar Gokce. Results showed that both fresh and dry weights of plants increased while real and relative water contents of plants decreased under drought stress. There was an increase in both malondialdehyde (MDA) and total protein contents under drought stress. Furthermore, glutathione reductase (GR) and catalese (CAT) enzyme activity increased in drought treated plants whereas guaiacol peroxidase (POD) and superoxide dismutase (SOD) enzyme activity decreased. Moreover, contents of indole acetic acid (IAA) and abscisic acid (ABA) increased in all tissue parts while contents of salicylic acid (SA), gibberellic acid (GA) and jasmonic acid (JA) increased in specific plant tissue parts during drought treatment. In conclusion it is obvious that all of these characters play essential roles in the drought tolerance of plants.Master Thesis Proteomic Basis of Drought Tolerance in Chickpea(Izmir Institute of Technology, 2010) Şelale, Hatice; Frary, AnneIn this study our aim was to identify differentially expressed proteins in root and leaf samples of the drought tolerant chickpea cultivar Gokce using proteomics approaches. For this aim we carried out 2D gel electrophoresis from total proteome extracts of root and leaf samples of Gokce cultivar from drought treated and control samples. In root 2D gels we obtained approximately 430 proteins; 14 of them were newly formed and 4 of them were disappeared in drought stress. Also we obtained 12 over-expressed protein and 4 down-regulated spot as a result of drought stress. In leaf 2D gels we obtained approximately 450 proteins 4 of them were newly formed spots, and 3 of them were disappeared in drought stress. For these samples we obtained 24 over-expressed proteins and 17 down-regulated proteins in drought stress. We identified differentially expressed proteins in MALDI-TOF/TOF mass spectrometer via peptide mass fingerprinting. Identified proteins are zinc finger (C2H2 type, AN1-like) family protein, pathogenesis-related family protein, STRS2 (STRESS RESPONSE SUPPRESSOR 2), 26S proteasome non-ATPase regulatory subunit 3, pentatricopeptide repeat-containing protein, RABB1C (ARABIDOPSIS RAB GTPASE HOMOLOG B1C); serine hydroxymethyltransferase, fiddlehead protein, aluminum-activated malate transporter, phloem protein 2-A8, ribosomal protein L30 family protein, N-rich protein with known function and we identified 14 hypothetical proteins with unknown function. Identified proteins are WRKY DNA-binding protein 6, myb family transcription factor, porin family protein, pentatricopeptide repeat (PPR) protein and transmembrane protein and 2 hypothetical proteins with unknown function.