Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Characterization of World Spinach Genetic Collection by Using Molecular Markers(Izmir Institute of Technology, 2015) Göktay, Mehmet; Frary, AnneSpinach (Spinacia oleracea L.) belongs to the Amaranthaceae family and is a popular and nutritious vegetable. It is believed that this green leafy plant originated from Persia which is now modern Iran and neighboring countries. In this study we screened 176 spinach world collection germplasm accessions with 15 known SSR markers. The markers produced 58 bands with 57 identified as polymorphic. PIC values of the markers ranged between 0.01 and 0.44. Average PIC value was 0.28. Based on allele analysis with Darwin5 and STRUCTURE tools, 176 individual were clustered into three groups. The first cluster mostly consisted of accessions from Europe and USA and contained 69 samples. The second cluster mostly consisted of lines from Asia and neighboring countries and contained 89 samples. The third cluster did not represent any characteristic according to geographic region, thus it was called an intermixed cluster which contained 18 samples. The maximum genetic dissimilarity of spinach accessions was 0.551 and minimum was 0.019. Average genetic dissimilarity was 0.217. Moreover we sequenced S. oleracea L. cv. Universal nuclear genome via IIIumina MiSeq technology and genome assembly was performed to develop new spinach–specific SSR markers. As a result, 3853 SSRs were identified in the assembled genome and we successfully designed 3275 primer pairs for these identified SSR motifs. These newly developed SSR markers will be helpful to scientists who are interested in spinach genome diversity and breeding.Master Thesis Qtl Analysis for Fruit Traits in Solanum Pimpinellifolium Inbred Backcross Lines(Izmir Institute of Technology, 2010) Tamer, Nilüfer; Frary, AnneTomato is one of the most economically and nutritionally important crops. It contains antioxidants such as lycopene, phenolics, vitamins E, C, and .-carotene at high levels. These traits are of interest to consumers and plant breeders for their health-related contributions. The main aim of plant breeding is to improve agronomically relevant traits by combining characters from different parental lines or their relatives. Genetic markers reveal these characters and other genetic differences between organisms. In this study both health-related and agronomically important traits were phenotypically identified by using 120 BC2F7, BC2F8, and BC2F9 IBLs. Also the lines were genotypically identified using the BC2F10 IBL population. A total of 66 COSII and 11 COS markers were positioned on the IBL map. A total of 103 QTLs were identified. Of these QTLs, 25 loci were identified for antioxidant traits: total water soluble antioxidant capacity, vitamin C content, lycopene and phenolic content. In addition, 78 QTLs were identified for agronomic traits: fruit weight, fruit shape, fruit firmness, stem scar size, external and internal color, locule number, fruit wall size, and soluble solid content. For most of the antioxidant QTLs, alleles from the S. pimpinellifolium parent were favorable. This result indicates that S. pimpinellifolium can be used as a source of high nutritional traits in order to improve elite tomato lines.