Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Determination of Whey-Based Medium Requirements and Growth Characteristics for the Production of Yoghurt Starter Cultures(Izmir Institute of Technology, 2008) Soydemir, Elçin; Harsa, Hayriye Şebnem; Harsa, Hayriye Şebnem; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyYoghurt is an important dairy product which is produced by thermophilic starter cultures including; Streptococcus thermophilus and Lactobacillus bulgaricus. They grow synergistically in milk by acidifying milk, produce specific aroma compounds and lactic acid which impact the quality of the yoghurt. Imported cultures are widely used in dairy industry to stabilize the technological properties which cause a change in our natural flora and flavour. Starters from traditional yoghurts have to be preserved. Under this perspective, the aim of this study was to determine the medium requirements of mixed starter cultures for production of starter bacteria which has been isolated and characterized before.Determination of medium requirements were done in a whey-based media. Since bacteriophages were one of the most important problem in dairy industry, to prevent cultures from phage adsorption, media was treated with different salts which can bind free calcium ions. Salts were examined for cell viability and phage infection effects.From these studies Na2HPO4 + KH2 PO4 with 2% of medium addition was chosen as the most effective. Mineral requirements of the mixed cultures were determined. The best combination; 10, 5, 50 ppm for Fe2+, Mn2+, Mg2+ ions with 0,939 desirability were used for initial mineral content of medium. Fermentation constants for mixed cultures were determined. Pure culture fermentations and mixed culture fermentations were performed. According to results mixed culture inoculations gave higher maximum specific growth rates; 0,9188h-1 and 0,7323h-1 for cocci and bacilli while 0,855h-1 and 0,659h-1 were obtained for cocci and bacilli from single strain fermentations.Master Thesis Investigations on Aroma Profile of Artisanal Yoghurt Starter Cultyres(Izmir Institute of Technology, 2012) Baran, Ezgi; Harsa, Hayriye Şebnem; Harsa, Hayriye Şebnem; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyYoghurt is an important fermented milk product that is consumed widely around the world as a part of the diet. Yoghurt is produced by lactic acid fermentation using Streptococcus thermophilus, and Lactobacillus delbrueckii ssp. bulgaricus. The most important criteria in yoghurt production are the selection of artisanal yoghurt starter cultures to achieve the typical aroma and quality of the final product. The typical aroma of yoghurt is identified chiefly by acetaldehyde that is the most important for yoghurt aroma. The aim of this study is to determine artisanal yoghurt starter cultures that provide the most desirable aroma to yoghurt, in order to promote industrial production with such flavor. In the first part of this study, the influence of different aroma cultures addition on the aroma production properties in yoghurt products was investigated. Nine different aroma cultures were added into the starter culture combinations at two different inoculation ratios. Among all of the combinations, just one yoghurt combination was selected according to the highest acetaldehyde and also the highest sensory scores. According to the results, two batches of yoghurt were produced: Sample 1 containing only yoghurt starter cultures (Lactobacillus bulgaricus Y30 + Streptococcus thermophilus Y24), was used as control yoghurt; Sample 2 containing yoghurt starter cultures plus with selected aroma culture (P10), was used as yoghurt with aroma culture. Yoghurt samples were stored at 4 °C for 21 days. The pH, titratable acidity, total solids, fat, protein, syneresis, viscosity, aroma profile, lactic acid and lactose profile, proteolytic activity, microbiological characteristics and sensory characteristics