Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Author: search.filters.author.Yalçın, Şerife HanımPublisher: search.filters.publisher.Izmir Institute of Technology

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  • Master Thesis
    Colloidal Plexcitonic Nanocrystals
    (Izmir Institute of Technology, 2022) Yalçın, Şerife; Yalçın, Şerife Hanım; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
    Noble metal nanocrystals, especially gold and silver, which have attracted a great deal of attention due to the supporting of surface plasmon polaritons (SPPs), have been extensively investigated and studied. With recent developments in colloid chemistry, synthesis of noble metal nanocrystals with tunable optical properties in the visible region of the electromagnetic spectrum has become easier. Until now, noble metal nanocrystals (NPs) synthesized by using various synthetic methods, have a variety of shapes, such as bipyramid, rod, disk, prism, and ring, etc. In the strong coupling regime, SPPs supported by metal nanocrystals interact strongly with excitons of organic dyes, semiconducting quantum dots (carbon or perovskite quantum dots) to generate a new hybrid optical mode called plexciton (plasmon-exciton). Plexcitonic nanocrystals have received interest owing to their ease of synthesis, scalability, and ability to provide sub-wavelength confinement of incident light and offer promising applications. Plasmon–exciton interaction at nanoscale dimension can be improved by generating new plexcitonic nanoparticles with tunable optical properties, which may be utilized in critical applications such as nanolasers, sensors, nano-optics, solar cells, and light emitting diodes. Therefore, there has been a tremendous amount of interest in the synthesis of new plexcitonic nanocrystals having excellent optical and chemical properties. The main goal of this thesis is to synthesize new plexcitonic nanoparticles with tunable optical properties in the visible spectrum: (i) synthesis of different shaped colloidal monometallic and bimetallic nanocrystals, (ii) synthesis of new colloidal plexcitonic nanocrystals, (iii) synthesis of carbon quantum dots (CDs), (iv) coupling of excitons of CDs and SPPs on the silver thin film.
  • Master Thesis
    Identification and Detection of Cis-Platin Binding Proteins by Laser Induced Breakdown Spectroscopy
    (Izmir Institute of Technology, 2015) Kaya, İbrahim; Yalçın, Şerife; Yalçın, Şerife Hanım; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
    In this study, an all-optically designed laser plasma spectroscopic technique for rapid identification and detection of cisplatin-binding proteins on electrophoretic gel spots prior to molecular mass spectrometric analysis is demonstrated. For this purpose, human serum albumin, human apo transferrin and horse heart myoglobin standard proteins and protein extracts from HeLa cancer cells were subjected to; incubation with cis-platin solution for several hours. Then, non-reducing polyacrylamide gel electrophoretic separation was applied. Followed by the visualization of proteins in the gel by Coomassie Brilliant Blue staining technique protein spots on the gel were dried between two cellophane sheets and subjected to laser ablation by highly energetic laser pulses. In addition, prior to nr-SDS-PAGE separation cis-platin binding to standard proteins were monitored by ESI-MS with several measurements made in 24 hours of incubation time. Using a Nd:YAG laser at its second harmonic wavelength, 532nm, 10 Hz frequency and 10 ns pulse duration, a micro-plasma was created on dried gel spots. Resulting plasma emission light was collected with collection lenses and transferred to a spectrograph via fiber optic cable. An intensified charge coupled device (ICCD) detector enabled multielemental analysis of platinum binding protein samples. Platinum binding proteins were recognized from the prominent neutral emission line, Pt (I) at 273.3 nm, in a plasma formed by the focused laser pulses on the gel, just in the center or in the vicinity of the electrophoretic spot. Spectral emission intensity of Pt lines from LIBS data has been optimized with respect to laser energy and detector timing parameters. Optimization of LIBS experimental parameters have been studied on polyacrylamide gels soaked in cis-Pt solution for Pt signal. It has been shown that, LIBS is a suitable method for identifying Pt in proteins, in gel medium, with nanogram levels of detection capability. The technique was applied to HeLa (human cervical cancer cells) cells extract for the detection of Pt-binded HSA after standard addition of known amounts of protein.