Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Department: search.filters.department.Thesis (Master)--İzmir Institute of Technology, BioengineeringSubject: search.filters.subject.Phytochemicals

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  • Master Thesis
    Phytochemical Studies on the Mastic Gum of Pistacia Lentiscus Var. Chia Collected From Karaburun Peninsula and Bioactivities of Isolates
    (01. Izmir Institute of Technology, 2021) Bedir, Erdal; Bedir, Erdal; Ballar Kırmızıbayrak, Petek; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Pistacia lentiscus L. is an evergreen shrub or tree-like plant named as 'mastic tree'. The phytochemical studies of Chios mastic gum have revealed that it is a complex natural product comprising several organic ingredients like natural polymers, essential oils and triterpenic compounds. Triterpenes are the main components of mastic gum, and they are thought to be responsible for important biological activities such as anti-cancer, anti-ulcer, cytotoxic, antidiabetic, and anti-inflammatory. Within the scope of this thesis, mastic gum collected from the mastic trees (Pistacia lentiscus var. chia) grown on the Karaburun peninsula of İzmir was investigated to reveal its phytochemical composition and neuroprotective effects of the isolates. Isolation and purification studies utilizing chromatographic methods are followed by structural elucidation of the isolates by spectroscopic methods (1D NMR, 2D NMR, MS and X-RAY). Detailed analysis of the obtained spectra helped us to establish structures of 14 molecules possessing triterpenic skeleton, and two of them turned out to be undescribed compounds. The bioactivity of selected compounds was screened for their neuroprotective effects against H2O2 induced oxidative stress on differentiated and undifferentiated SH-SY5Y cells. Two compounds showed neuroprotective effects against H2O2-induced cell death on undifferentiated SH-SY5Y cells at 5 and 10 µM concentrations, while the other compounds either showed little or no effect. This study provides promising new insights into the use of mastic gum or its components in the treatment of neurodegenerative diseases.
  • Master Thesis
    Extraction of Phytochemicals From Scolymus Hispanicus and Determination of Potential Health Effects
    (Izmir Institute of Technology, 2017) Ahmad, Bilal; Büyükkileci, Ali Oğuz; Güleç, Şükrü; Büyükkileci, Ali Oğuz; Güleç, Şükrü; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Golden thistle (Scolymus hispanicus) is an edible medicinal plant growing in Turkey. It has been in use since decades for the treatment of various disorders by local folks. In the past the extracts from the root barks of S. hispanicus were in use in the form of a medicine for the removal of kidney stones. Its root barks are the only eaten part and the root internal and aerial parts are considered as residues. The effect of harvesting time and plant maturity on phytochemicals composition of this plant have not been studied before. Besides that, in previous studies only the edible part was analyzed for bioactive constituents and the residues (aerial parts and root internal parts) have not been analyzed before. In this study, various phytochemicals and total antioxidant activities in the ethanol extracts of aerial parts, root barks and root internal tissues of S. hispanicus harvested from November 2016 to July 2017 were measured. The dominant phytochemicals were different in the roots and the aerial parts, whereas phytochemicals were influenced differently by the harvesting time. Total phenol contents and total antioxidant activities were higher in the aerial parts than the root parts, while total triterpenoid contents were higher in root barks and root internal tissues. Thin Layer Chromatography (TLC) analysis showed that there were not any free triterpenoids in the extracts, however there were glycosides, which may have contained triterpenoids. The crude extracts of S. hispanicus showed cytotoxic effects on Caco-2 cells growth. The results suggested that these extracts might have potential preventative effects on colon cancer.