Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Investigation of Cytotoxic Properties of New Isoindol Derivatives in Lung and Cervical Cancers(Izmir Institute of Technology, 2019) Almusawi, Yasir; Şanlı Mohamed, Gülşah; Şanlı Mohamed, Gülşah; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of TechnologyCancer is one of the most common diseases in the world. Recently, there are many methods developed by researchers to treat this disease. One of these treatments is targeted for chemotherapy. It is preferred by researchers because it is less toxic and has fewer side effects than other cancer treatments. This study emphasizes the anticancer properties of the newly synthesized Isoindole derivatives. Thus, it was hoped to be a significant improvement based on new generation anticancer compounds with high efficacy and fewer side effects. The main objective of this study was to investigate the biological activity of seven newly synthesized Isoindole derivatives. The anticancer activity of these compounds was evaluated against HeLa (cervical carcinoma) and A549 (lung adenocarcinoma) cancer cell lines. This study is divided into three parts. Firstly, the cytotoxic activity of these compounds was determined by measuring the cell viability of each compound on HeLa and A549 cell lines. The main objective of this analysis is to measure the IC50 value of each compound and determine which compound is best to kill at least half of the cells. Secondly, the effects of programmed cell death and cell cycle were investigated for compounds with the best IC50 for each cell line by using Annexin V-FITC in flow cytometry. Finally, a scratch assay was performed to investigate the effect of these new Isoindole derivatives on cell migration.Master Thesis Investigation of Stilbene-Fused Chalcone and Flavanone Derivatives for Their Cytotoxic and Anti-Cancer Properties(Izmir Institute of Technology, 2012) Odacı, Burcu; Çağır, Ali; Çağır, Ali; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of TechnologyStilbene, chalcone and flavanones are three major classes of molecules, which can be found in plants as secondary metabolites. Derivatives of those may possess variety of biological activities. In this study it is aimed to synthesize a hybrid molecule which may show the biological activities of both flavanone and stilbene, or chalcone and stilbene simultaneously. For this purpose previously synthesized 11 simple chalcone, flavanone and stilbene derivatives and 31 stilbene-fused chalcones and stilbene-fused flavanones were tested for their cytotoxic activities in prostate cancer cell line (PC-3) and breast cancer cell line (MCF-7) by using MTT assay. Then aromatase inhibition properties of simple chalcones, flavanones, stilbenes, stilbene-fused chalcones and stilbene-fused flavanones were studied. Results of the study were evaluated in potential of the hybrid system to carry out more than one biological activity and mimicking performance of the simple ones. Results indicate that tested simple chalcone and flavanone derivatives are more cytotoxic than simple stilbenes in both cancer cell lines. On the contrary, simple stilbene structures were much more successful in aromatase inhibition assays. Cytotoxic activity profiles of stilbene-fused chalcones in cancer cells show that those molecules mostly mimic the simple chalcone structures. On the other hand, flavanones lost their cytotoxic activities when they were fused with stilbenes. In addition, aromatase inhibition assay showed that stilbene-fused chalcones again do mimic the simple chalcones but not simple stilbenes. In the same assays, stilbene-fused flavanones may mimic both simple flavanones and simple stilbenes by depending on the type and position of the substituent in terminal aromatic rings.