Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Characterization of Pollen-E1 Gene Might Play Role in Salt Tolerance in Beta Maritima(Izmir Institute of Technology, 2015) Uysal, Özge; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologySalinity stress has a negative impact on the growth of plants, which affects homeostasis and productivity. The uptake of non-essential salt ions change the osmotic balance of the cell and cause dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. In this project, we isolated and characterized salt tolerance genes in Beta maritima plant. For this purpose, functional genomics technique was used by over expressing cDNAs in yeast and colonies can grow toxic salt media isolated and characterized. We found several colonies and we focused on uncharacterized Pollen-E1 gene with an unknown function. Pollen-E1 cDNA confers salt tolerance to yeast cells. Intracellular sodium measurements of Pollen-E1 overexpressed in yeast cells showed decreased salt levels as compared to wild type suggesting that sodium was transported out of the cell. Pollen-E1 protein localized in endomembrane systems in the yeast cells. In mRNA expression analysis, Pollen-E1 mRNA levels induced immediately in leaves and later stages in root systems under salt stress. Our results showed that is the uncharacterized and unknown function Pollen-E1 gene might have some role of regulating salt tolerance in Beta maritima.Master Thesis Construction of Cdna Library From Hordeum Marinum To Indentify Salt Tolerance Genes(Izmir Institute of Technology, 2015) Alkaya, Naki; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologySalt is necessary for plants because of ion homeostasis; however, excess uptake of salt leads to damage in plant cells, which may even result in the death of the plant. Hordeum marinum, also known as sea barley, is a member of Poaceae family that resides in coastal areas, so it is thought that it may have a possible salt tolerance gene or genes. Therefore, this study aims to identify the genes involved in salt tolerance in Hordeum marinum by functional genomics method. After screening, seven transformant yeast colonies found and sequence analyses of these plasmids gave homology to hypothetical protein of Bipolaris oryzae. To confirm salt tolerance of this protein, salt sensitive yeast cell transfected by this candidate gene was checked in high salt concentration containing medium. Based on solid growth assay, these transgenic yeast cells could survive in 1M saline medium. Hence, it is hypothesized that Hordeum marinum and Bipolaris oryzae might have a symbiotic association. It is possible that in this association Bipolaris oryzae may play a role as endophytic fungus that might also confer salt tolerance in Hordeum marinum.