Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Association Mapping for Oil and Protein Content in Hazelnut (corylus Avellana L.)(Izmir Institute of Technology, 2020) Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyEuropean hazelnut has an important place in terms of nutritional and economic value among tree nut species. Because of its nutritional content, the consumption of hazelnuts promotes human health in many ways. These nutritional components are controlled by multiple genes and affected by the environment; therefore, they are quantitative traits. The vast majority of world hazelnut production is provided by Turkey. So it is important to develop hazelnuts with higher nutritional quality for our country. In this work, we aimed to associate genetic diversity data and oil and protein content of hazelnuts to identify QTL. For this purpose oil and protein content were measured in kernels of 96 accessions. Genotypic data were obtained with 30 SSR markers and resulted in 407 polymorphic alleles. According to allelic data, the mean dissimilarity value was 0.52 (52%) for the 96 accessions. Population structure analysis resulted in three clusters with 30, 30, and 16 accessions. Twenty accessions could not be assigned to any cluster and were considered admixed. Association mapping between allelic and phenotypic data indicated that five loci were significantly associated with oil content. The most significant result for oil content belonged to B628-307 loci (p=0.0002, r2=0.145). Three loci were detected for protein content. Among them, A613-153 had the most significant effect (p=0.003, r2= 0.088). We hope that our survey of germplasm and the identified loci associated with oil and protein amount can accelerate hazelnut breeding. In the future this study can contribute to develop new genotypes with enhanced nutritional value.Master Thesis Pretreatment Methods for Valorization of Hazelnut Pruning Wastes(Izmir Institute of Technology, 2016) Doğru, Kevser; Büyükkileci, Ali Oğuz; Büyükkileci, Ali Oğuz; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyTurkey is the world leader in hazelnut production and a large amount of residues is produced during its harvesting and processing. So far, the residues of hazelnut production had no economic value and usually burned in the fields. Obtaining valuable products such as ethanol from hazelnut pruning waste (HPW) can add value to those. Ethanol produced by microorganisms via fermentation is a promising alternative biofuel. Ethanol has been produced for a long time from sugary substances, while lignocellulosic biomasses (LCBs) are interesting alternative to fossil fuel based resources in order to have a sustainable production process. Liquid hot water (LHW) treatment is one of the pretreatment processes necessary to facilitate enzymatic hydrolysis of cellulose into glucose before ethanol fermentation. Organosolv is similar to LHW treatment except that ethanol-water mixture is generally used is the liquid part instead of only water. LHW could remove the hemicelluloses from the lignocellulosic matrix to some extent, while adding H2SO4 improved the hemicellulose removal. Organosolv was effective on removal of lignin as well as of hemicellulose. Acid catalysis improved the hemicellulose solubilization in organosolv, like in LHW treatment. After acid catalyzed organosolv, cellulose content of the HPW was increased to 67.91%. This sample was hydrolyzed with a conversion efficiency of 87.32%. Hydrolysate containing 60.63 g/L glucose was used as the medium for ethanol production using Saccharomyces cerevisiae. At an 83.49% theoretical yield, 22.2 g/L ethanol was obtained after 6 h. These results demonstrated that hazelnut pruning waste has potential to be used as a feedstock for ethanol production.