Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
Browse
2 results
Search Results
Master Thesis Characterization and Utilization of Injectable Hydrogels for Tissue Engineering Applications(Izmir Institute of Technology, 2020) Güzelgülgen, Meltem; Arslan Yıldız, AhuTissue engineering combines the knowledge of the engineering aspects with life sciences to improve human health. Recent studies in tissue engineering have focused on investigating biocompatible scaffold materials and design. Quince seed hydrogel(QSH) has been used in traditional and modern medicine for skin wound and burn treatments, synovial lubrication, cough and asthma removal, and oral drug delivery with its antioxidant potential and biocompatible aspects. This thesis focuses on developing QSH and evaluating its potential as an injectable hydrogel in treating bone tissue defects as a totally new tissue scaffold and also as a promising tissue filling material. For this purpose, QSH scaffold optimization was carried out using various concentrations of hydrogel and crosslinkers which were glutaraldehyde(GTA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)/N-hydroxysuccinimide(NHS). Morphological and chemical analysis of QSH was done using SEM, FTIR, AFM, and protein adsorption test. Thus, porosity, swelling ratio, degradation rate and surface characteristics were evaluated. NIH-3T3 and SaOS-2 cell lines were utilized for 3D cell culture formation. Afterward, 3D spheroids were analyzed for cell viability and proliferation by using AlamarBlue and LiveDead assays, and also cell imaging technics. Results showed that QSH scaffolds did not show any cytotoxic effect on NIH-3T3 and SaOS-2 cells. The optimum results were achieved with 2mg/mL of QSH and 0.03M GTA concentrations; where 76.59µm average pore size, 56.8 fold water holding capacity and at least 80% cell viability was observed. Therefore, it was concluded that QSH has a high potential to promote tissue engineering applications with its injectable texture as a filling material.Master Thesis Hydrogels and Self-Assemled Nanostructures Based on Wool Keratose(Izmir Institute of Technology, 2017) Pakkaner, Efecan; Top, AybenIn this study, water soluble keratose proteins were extracted from “Ovis aries” wool using peracetic acid oxidation with a yield of 35 ± 5 %. Wool samples and the extracted keratose proteins were characterized by using FT-IR, XRD, SEM and TGA techniques. α-keratose fractions (MW = 43-53 kDa) along with cleaved fragments of α-keratoses with molecular weights between 23 and 33 kDa were identified in the extracted protein mixture using SDS-PAGE analysis. DLS and AFM experiments indicated self-assembled globular nanoparticles with diameters of 20-40 nm formed at 5 and 10 mg/ml keratose concentrations. On the other hand, at 10 % w/v keratose concentration interconnected keratose hydrogels with pore sizes of 6 ± 4 and 7 ± 4 μm were obtained upon incubation at 37 and 50 °C, respectively. Storage moduli (G’) of these physical hydrogels were increased from ~100 to ~1000 Pa, as gelation temperature was increased from 37 to 50 °C. Hydrogels were also obtained at 7.5 % w/v keratose concentration by the addition of a crosslinker, THPC. Amine group:crosslinker ratio was used as 1:1, 1:2 and 1:4. As the amount of crosslinker increased, network transformed from fibrous to more planar structures exhibiting a significant decrease in average pore size from 24 to 11 μm. G’ values of the crosslinked hydrogels were obtained between ~1 and ~5 kPa tuned by the crosslinking amount. Cell interaction properties of a select physical hydrogel prepared at 37 °C was tested using CCK-8 assay. It was observed that the keratose hydrogel supported L929 mouse fibroblast cell proliferation as much as collagen, which suggests that these keratose hydrogels can be promising candidates in soft tissue engineering applications.