Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Antimicrobial, Antioxidant, Antiproliferative and Cytotoxic Activities of Aronia Fruit Extract(01. Izmir Institute of Technology, 2023) Tanağardı, Dilaranur; Baysal, Ayşe Handan; Sezgin, EfeAronia melanocarpa contains high amounts of phenolic compounds, especially anthocyanins. Because of its high chemical content and significant antioxidant action, this food is known as a functional food, and its use is spreading worldwide. Within the scope of this thesis research, studies were carried out on Aronia melanocarpa dry extract and liquid extract. For these purposes, chromatographic and chemical profile were determined in detail by HPLC (High-Pressure Liquid Chromatography), Fourier-Transform Infrared Spectroscopy (FTIR) and Q-TOFF- MS (Quadrupole Time of Flight Mass Spectrometry), and significant bioactive were determined. Spectroscopic methods were used to characterize phenolic, anthocyanin, and flavonoid components. Aronia melanocarpa dry and liquid extracts were tested for antioxidant activity using DPPH and ABTS methods. The antioxidant potential of the Aronia melanocarpa dry and liquid extracts studied is high. To test the antiproliferative and cytotoxic effects, cytotoxic studies were performed on the CaCo2 cell line. Cell migration was also studied in HUVEC and HaCat cell lines. The antimicrobial activity of Aronia melanocarpa dry extract and liquid extract was tested against Saccharomyces cerevisiae, a yeast species, and bacterial strains of Staphylococcus aureus, Escherichia coli O157:H7, Salmonella Typhimurium. The extracts analyzed showed an antimicrobial effect on the tested bacteria at different concentrations. The results obtained in this study emphasize that the tested Aronia melanocarpa dry extract and liquid extract have antimicrobial properties. Chromatographic, chemical, and cytological data reveal that Aronia melanocarpa liquid and dry extracts can be used as antioxidative and antiproliferative products as food supplements in the health field.Master Thesis Investigation of Anticancer Properties of the Novel Synthesixed Pyrrole Derivatives as Potential Tyrosine Kinase Inhibitors(Izmir Institute of Technology, 2017) Kaya, Meltem; Şanlı Mohamed, GülşahIn cancer treatment, chemotherapy has some serious side effects, because it targets active cells which might not be cancer cells. Mouth, hair, nail, bone marrow cells are some examples of active cells. For the reason that chemotherapy has side effects, targeted therapy become more important. Tyrosine kinases are most interested target, because they are necessary for cell growth and metastasis. Active form of tyrosine kinases can cause tumour growth and proliferation, angiogenesis, metastasis and antiapoptotic effects. Based on these vital role of tyrosine kinases, they became more important target in cancer treatment. Pyrrole derivatives have been used chemotherapy drugs for years. Semaxanib and Sunitinib, indole derivatives, are tyrosine kinase inhibitors. The main purpose of this research is to investigate the biologic activities of novel synthesized seven pyrrole derivatives, their activities on migration, apoptosis, cell cycle, and mTOR downstream as a potential tyrosine kinase inhibitor. The results of this research proved that these seven compounds have toxicity on HeLa cells with the IC50 values of 140.60 μM, 382.82 μM, 366.44 μM, 542.00 μM, 255.86 μM, 148.59 μM, 171.40 μM, respectively, but toxicity effects of drugs do not depend on apoptosis mechanism. Beside this, D1 and D3 were able to effect cell cycle by arresting at S phase for D1 and G1 phase for D3. It was demonstrated that D1 and D3 inhibited cell migration. And this inhibition was reported as in a relation with overexpression of p- 4EBP1, inhibition of p-p70S6K (Thr) and p-p70S6K (Shr) proteins. Considering all results, D1 and D3 might be potent inhibitory of metastasis of HeLa cells with respect to its effect on cell cycle, migration, p-4EBP1, p-p70S6K (Ser), and p-p70S6K (Thr) protein levels.