Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Correlation of Genetic and Microbial Changes in Inflammatory Bowel Disease(01. Izmir Institute of Technology, 2021) Terlemez, Gamze; Sezgin, EfeInflammatory bowel disease (IBD) is complex, multifactorial, polygenic diseases without a certain cure. Patients require lifelong surveillance, expensive treatments, risky surgeries, have increased cancer and other systemic disease risks. IBD consists of two groups as Ulcerative colitis (UC) and Crohn's disease (CD). Although pathogenesis of IBD is not clear, a dysregulated immune response to environmental factors (intestinal microbiota) develops in genetically susceptible individuals. As early diagnosis is hard, most patients are diagnosed at a later stage, however, they suffer from colon damage and lose the chance of early treatment. This thesis aimed to identify biomarkers that can analyze IBD patient risk profiles to control disease and reveal individuals at risk before the disease progresses further. Firstly, mutations in Turkish IBD patients were investigated in candidate regions of innate immunity (NOD2), adaptive immunity (IL-23R), and autophagy (ATG16L1) genes. Then, microbiota profiles of patients were revealed by NGS and qPCR. Finally, to identify relationship between mutations and microbiota, statistical analyses were performed with non-parametric methods. As a result of this study, Shannon index showed significant reduction of bacterial diversity in IBD patients versus the control group (p-value, 0.048). At the species level, relative abundance of F. prausnitzii decreased while abundance of E. coli increased in IBD patients. Significant relationship (p-value, 0.034) was found between rs11209026 genotype and F. prausnitzii reduction in CD but not in UC. In conclusion, converting significant observations into a molecular test kit can provide a molecular guide that helps gastroenterologists in planning personalized treatments in IBD patients.Master Thesis Fabrication of Thin Layer Polymer-Based Biointerphase for Biosensing Application(İzmir Institute of Technology, 2016) Yücel, Müge; Yıldız, Ümit HakanThis study aims to fabricate polymer-carbon nanotube composite as a bioelectronic interface for sensing volatile organic compounds (VOCs) in exhaled breath. Sensor platform is made of two layers i) polymeric membranes and ii) conducting layer. Poly(vinylidene fluoride) (PVDF), polystyrene (PS), and poly(methyl methacrylate) (PMMA) are selected as model polymers that are processed by electrospinning to utilize polymeric membranes. Multi-walled carbon nanotubes (MWCNTs) are used to fabricate conducting layer on top of PVDF, PS, PMMA polymer membranes. Aqueous solution of well-dispersed MWCNTs are obtained by several purification and filtration steps and conductivity of working MWCNT solution is adjusted about 120 μS/cm for whole study. This solution is further used to impregnate PVDF, PS, PMMA membrane. The PVDF-MWCNT, PS-MWCNT and PMMA-MWCNT sensor platforms are tested by electrochemical station that recording electrical resistivity change by time. All sensors platforms, made of three polymeric membranes-MWCNT, are found to be a responsive upon applying the toluene and acetone vapor. The sensing mechanism is hypothesized as the adsorption of VOCs onto the conducting CNT layer blocking electron stream on CNT network and causing resistivity change. The sensitivity of PVDF-MWCNT sensing platform is exceedingly higher with respect to other two candidates due to solvent vapor- polymeric membrane interactions. This contribution changes sensor platform characteristics and make them quite sensitive to trace amount of VOCs. Acetone and toluene are detected from ppm to ppb range and reproducible responses are recorded. As a result, acetone and toluene, biomarkers of diabetes and lung cancer, can be differentiated with produced sensor.