Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Subject: search.filters.subject.Olive leaf extraction

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  • Master Thesis
    Synthesizing of Olive Leaf Exteact Loaded Calcium Alginate Chitosan Microcapsules and Investigation of Their Anticancer Activity on Cancer Cells
    (Izmir Institute of Technology, 2014) Bal, Yıldız; Şanlı Mohamed, Gülşah; Bayraktar, Oğuz; Şanlı Mohamed, Gülşah; Bayraktar, Oğuz; 03.02. Department of Chemical Engineering; 04.01. Department of Chemistry; 03. Faculty of Engineering; 04. Faculty of Science; 01. Izmir Institute of Technology
    As we all know there are too many diseases which can be counted as fatal. But cancer is one of the most terrifying one for human race. Moreover, breast cancer in females and lung cancer in males are the most common cancer types. Although there are so many treatment methods, they have severe side effects. On the other hand, mother earth is so willing to give her hand and open all of the sources she got. O. europaea is one of the most important valuable source and Mediterranean area is blessed with that source. Olive and olive products may play an important role in cancer prevention. However, there are some limitations on their direct usage. Since encapsulation technology can seal valuable things into small capsules, it can be used to alleviate these limitations. In this study, the aim was figure the limitations of olive leaf extract (OLE) out with the encapsulation technology and enhance the effectiveness. Moreover, assess this effectiveness on cancer cell lines. Thus, OLE loaded calcium alginate (OLE-Ca-Alg) capsules produced by ionic-crosslinking. To limit the loss of OLE, capsules were coated with chitosan (CS) and OLE loaded chitosan calcium alginate (OLE-CS-Ca-Alg) were produced–two stage procedure. Optimization studies and characterization of the microcapsules were carried out. To investigate their anticancer effectiveness cytotoxicity, cell cycle and apoptosis analysis were performed for lung and breast cancer cell lines besides cytotoxic effects of the capsules were compared with healthy cell line. Additionally, visual observations were done by optical microscopy. Consequently, results showed OLE loaded capsules are more cytotoxic than free OLE. These effects were supported with cell cycle, apoptosis analysis and optical microscopy observations. In contrast none of compounds was cytotoxic for healthy cells. Thus, it can be concluded that our results will enhance the drug industry as it is a new approach for anticancer drugs and biocompatible material for biomedical applications.
  • Master Thesis
    Immobilization of Olive Leaf Extract on Chitosan Nanoparticles and Investigation of Their Effects on Cancer Cell Lines
    (Izmir Institute of Technology, 2014) Özdamar, Burcu; Şanlı Mohamed, Gülşah; Şanlı Mohamed, Gülşah; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
    Cancer incidence and mortality rates are increasing worldwide in both economically developed and developing countries. Breast cancer in females and lung cancer in males are the most common cancer types. Epidemiological research has provided increasing evidence that dietary habits, especially Mediterranean diet which has high consumption of olive oil and its products, may play an important role in lung and breast cancer. Due to their preventive effect against cancer, olive leaf extract rich in polyphenols was immobilizied on chitosan nanoparticles which are good drug carriers because of their biocompatible and biodegradable properties with the help of capability of passing through biological barriers. For this aim, olive leaf extract loaded chitosan nanaoparticles were synthesized by ionotropic gelation mechanism. Optimum conditions to synthesize nanoparticles were determined by investigation of the effect of chitosan and tripolyphosphate mass ratio, initial pH of chitosan solution, concentration of olive leaf extract and incubation time of olive leaf extract and tripolyphosphate with chitosan solution. Characterization of nanoparticles was performed by dynamic light scattering, atomic force microscopy and infrared spectroscopy. To investigate the anticancer properties of nanoparticles, molecular biological studies were performed by in vitro cytotoxicity studies based on MTT assay, in vitro cell cycle analysis and apoptosis by flow cytometer and imaging of cells by optical microscopy. In results, olive leaf extract loaded chitosan nanaoparticles obtained approximately 91.25 nm and showed more cytotoxicity than chitosan nanoparticles, chitosan and olive leaf extract for both lung and breast cancer cells. In contrast, there was no cytotoxicity for healthy cells. These effects were supported by cell cycle analysis. Also in optical imaging, lower number of cells and morfological differences on cancerous cells which supports the cytotoxicity results were observed. We can conclude that our results will open a new approach to use not only cytotoxic anticancer drug for cancerous cells but also biocompatible material for biomedical applications.