Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Molecular Mapping of N Gene Conferring Resistance To Root-Knot Nematodes in Pepper(Izmir Institute of Technology, 2013) Arslan, Mehmet Enes; Doğanlar, Sami; Doğanlar, SamiPepper (C. annuum) is one of the most important agricultural crops worldwide and Turkey ranks third among all countries in pepper production. Pepper species have economical and also pharmaceutical importance so, it is vital to develop different methods to increase pepper yields. The root-knot nematode (Meloidogyne species) is one of the most important biotic factors that affect pepper growth and development in Turkey. The dominantly inherited N gene which was mapped on chromosome P9, 7 cM from Me1 and 2 cM from Me3, confers resistance to pepper species against Meloidogyne species. The aim of this work was to develop a marker tightly linked to the N gene which can be used in marker-assisted selection. A total of 132 SSR Hpms primers, 230 EST-SSR markers and 45 chromosome 9 specific primers were used to a construct linkage map and find an N linked marker. Hpms SSR markers gave 19% polymorphism by capillary electrophoresis, EST-SSR markers showed 5.2% polymorphism by agarose gel electrophoresis while the chromosome 9 specific markers, yielded 20% polymorphism by fragment analyzer. When all 407 analyzed markers are considered, only 11.3% polymorphism was observed and these results were expected because we used an intraspecific population. The, polymorphic markers were mapped in a "Carolina Wonder" X "AZN-1" F2 population and analyzed with JoinMap software. Three markers were linked with the N gene. These markers are ScarPM6a (3.6 cM), ScarPM6b (10.2 cM) and ScarN (22.6 cM) which are located with same segregation group with the N gene. These markers will allow development of a marker tightly linked to the N gene which can be used in marker-assisted selection to increase the efficiency and effectiveness of pepper breeding for nematode resistance.Master Thesis Development of Molecular Markers Linked To Me1 Gene Conferring Resistance To Nematode in Pepper(Izmir Institute of Technology, 2012) Öztürk, Leyla; Doğanlar, SamiPepper (Capsicum annuum L.) belongs to family Solanaceae. It is an agronomically important plant originating from Mexico. Pepper yield, quality and growth are limited by plant nematode parasitism. Root-knot nematodes (Meloidogyne spp.) are the most damaging sedentary endoparasites. M. incognita race 2 is the most common root-knot nematode found in Turkey. The Me1 gene which confers resistance to M. incognita was mapped in pepper to a 28 cM interval on chromosome 9. The aim of this study was to develop molecular markers linked to this nematode resistance gene. Phenotypic characterization of nematode resistance was performed for 200 F2 individuals from the cross between resistant pepper cultivar PM217 and Turkish susceptible cultivar AZN-1. In the F2 individuals, 151 plants (76%) were evaluated as resistant, 49 plants (24%) were susceptible. Markers were tested on parents of the mapping population to identify polymorphisms. A total of 30 standard markers and 548 new marker combinations were tested. Of these 578 markers, 75 (13%) were polymorphic. They were applied to F2 population and 28 (37%) showed clear segregation on F2 population. Eighteen of the markers (64%) segregated dominantly, 10 of the markers (36%) segregated codominantly. Markers located near Me1 on chromosome 9 were used for the construction of a linkage map. Out of three markers, SCAR_CD was the nearest marker to Me1 gene with a distance of 1.1 cM. These markers will provide selection at the genotypic level by marker-assisted selection, which will increase the efficiency and effectiveness of pepper breeding for nematode resistance.