Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Characterization of Glycoalkaloid Content and Molecular Mapping in Eggplant(Izmir Institute of Technology, 2006) Kırsoy, Öyküm; Frary, Anne; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyIn this thesis, solamargine which is a known eggplant glycoalkaloid and has an important place for human health was characterized in eggplant. For characterization, two eggplant lines S.melongena MM738 and S.linnaeanum MM195 were used. Although, for identification and detection of glycoalkaloid concentration, many different methods have been utilized, for this thesis, high-performance liquid chromatography (HPLC) was used to analyze glycoalkaloid concentration in eggplant. In HPLC, spiking of samples was done using a solamargine standard and it was found that S. melongena had an undetectable level of solamargine while S. linnaeanum had between 17.6 and 33.4 mg (average 25.5 ± 11) solamargine per gram of freeze dried powder. In addition to characterization of glycoalkaloids in S.melongena MM738 and S.linnaeanum MM195, different types of molecular markers were surveyed for polymorphism in S.melongena MM738 and S.linnaeanum MM195 for mapping. A total of 47 polymorphic markers were then tested on the F2 population and located on the eggplant molecular genetic map.Master Thesis Genetic Mapping and Characterization of Eggplant for Glycoalkaloid Content(Izmir Institute of Technology, 2010) Gürbüz, Nergiz; Gürbüz, Nergiz; Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyMost plants including eggplant, produce toxins against insects, pathogens and animals. The biggest class of these toxins is alkaloids. Generally plants produce alkaloids in a glycosidic form which are called glycoalkaloids. Glycoalkaloids have toxic effects on human health. For example, as a result of disrupting the cell membrane, the gastrointestinal track and other organs are damaged. Glycoalkaloids are not only toxic to human health but also they have beneficial effects. For example, they decrease cholesterol level, and have anticancer activity. And also they are used as a precursor for steroidal drugs. Therefore, isolation and determination of glycoalkaloids content is important. For this aim, column chromatography was performed and solamargine and solasonine were obtained. Totally 122,8 mg solamargine, solasonine and solamargine and solasonine mix from Solanum linnaeanum were obtained. Their identities were confirmed via 1H NMR and mass spectroscopy. For determination of glycoalkaloid content HPLC method was developed via changing different parameters such as mobile phase, pH, temperature, flow rate and column type. It was found that separation of glycoalkaloids by using RP column without phosphate buffer was not sufficient. On the other hand, separation of glycoalkaloids by using carbohydrate column was better but this type of column is not stable. Furthermore, linkage mapping was performed by using CAPs and SSR methods; and 385 COSII markers and 221 SSR markers were tested. Of the tested markers, 38 of the COS II markers and 28 of the SSR markers were found to be polymorphic. A total of 52 of these markers were mapped.Master Thesis Determination of Genetic Diversity Between Eggplant and Its Wild Relatives(Izmir Institute of Technology, 2007) Tümbilen, Yeliz; Doğanlar, Sami; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyEggplant (Solanum melongena L.) is an important crop and has a growing reputation and is now cultivated globally. It is a valuable member of the human diet in Asia, especially in India, which is a primary diversity center of the species. Turkey is the first in Europe and is in the first five countries around the world in terms of eggplant production. The Solanaceae family to which S. melongena belongs is an important family, too. Tomato, potato, tobacco and petunia are some example species of the Solanaceae family. This important family with 3000-4000 species shows a high level of morphological diversity which results in confusion about its systematics and this diversity is at the level of genera, species and cultivars. The aims of the studies reported in this thesis were to analyze genetic diversity of Turkish eggplants and wild relatives in separate studies with different molecular tools. To reveal genetic diversity among eggplant cultivars grown in Turkey, the AFLP marker system was applied to the sample genotypes. For the investigation of genetic variation between S. melongena and its wild relatives, though, the SSR marker system was used. For the AFLP data for Turkish eggplants, an r value of 0.97 was obtained which was in the best scale. Eigen values reported here were also informative. These results showed that the first component analysis explained 64.34% of the variation between samples. For three axes, though, a total of 72.21% variation was explained. According to the statistical results of SSR analysis, the r value of Solanum species. genotypic data was found to be 0.88. That means the correlation between sample genotypic data and dendrogram was found to be high. Due to the other statistical results which were Eigen values explained 46.12% of genotypes for first component analysis. With a total value of 55.28%, the 47 different genotypes were explained by the three principle component axes. The results of AFLP studies showed that although a high similarity value was observed, diversity was detectable among the accessions. The results of SSR studies were also meaningful with their concordance with previous studies and observed diversity with a good fit to statistical results.