Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Determination of Genetic Diversity and Population Structure in Faba Bean (vicia Faba L)(Izmir Institute of Technology, 2015) Göl, Şurhan; Frary, Anne; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyFaba bean (Vicia faba L.) is an important legume species because of the high protein and starch content of its seeds. Broad bean can be grown in different climatic conditions and is an ideal rotation crop because of the symbiotic relationship between the plant and nitrogen fixing bacteria in its roots. Broad bean seeds are consumed as fresh vegetables in many countries throughout the world. However, the genetic diversity found in this germplasm has not yet been characterized and has not been systematically used in broad bean breeding programs. In this project, faba bean individuals obtained from International Center for Agricultural Research in the Dry Areas (ICARDA), Centre for Genetic Resources (CGN), Aegean Agricultural Research Institute (AARI), Nordic Gene Bank (NGB) and Australia (The University of Adelaide, Jeffrey Paull) were examined for their genetic diversity and population structure. For this purpose, 259 faba bean germplasm accessions were characterized using 32 SSR primers. A total of 302 polymorphic SSR fragments were analyzed. According the results, faba bean individuals were divided into two main clusters based on Neighbor-joining algorithm (r = 0.9062) with some clustering based on geographical origin as well as seed size. STRUCTURE 2.2.3 program was used to determine population structure. K was determined as 2 subpopulations. Cluster 1 had 87 individuals; cluster 2 had 162 individuals and 10 individuals were intermixed with results generally agreeing with the dendrogram analysis. A total of 45 well-characterized faba bean individuals were selected for the core collection to be used in breeding studies.Master Thesis Determination of Genetic Diversity in National Olive Collection (olea Europaea L.) Using Ssr and Srap Markers and Development of Snp Marker for Traceability of Memecik Olive Cultivar(Izmir Institute of Technology, 2010) Işık, Neslihan; Frary, Anne; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyIn this study genetic diversity of the Turkish olive collection was successfully characterized by using 13 SSR and 12 SRAP markers. For SSR marker analysis, we also added 3 European accessions to the national cultivars and binominal microsatellite data were detected by Qiaxcel software and clustered by NTSYS program. The UPGMA dendrogram showed good fit with the distance matrix (r . 0.85). While the outgroups were clustered with 0.27 similarity to national accessions, Turkish accessions clustered with 0.55 minimum similarity. Using SRAP markers we found 0.66 minimum similarity among Turkish accessions with a good fit between the distance data and UPGMA dendrogram (r . 0.83). The SSR and SRAP distance matrices were compared and they were found to correlated at r .0.23. To support the tree, ordination tests (PCA) for each marker system were performed and similar clustering was seen as in the trees. The other aim of this research was to develop SNP markers for traceability of Memecik accessions. We sequenced the anthocyanidin synthase gene of 11 exported olive accessions and aligned using Biolign program. There was no unique SNP that could be used to separate Memecik from the other accessions so we used a combination of SNPs. By using two probes at the same time, Memecik accession was distinguished from the other 10 exported accessions. In addition to find the SNP marker to trace Memecik accession, we developed the SNP marker to trace Trabzon Yağlık accessions.