Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
Browse
3 results
Search Results
Master Thesis Construction of Cdna Library From Hordeum Marinum To Indentify Salt Tolerance Genes(Izmir Institute of Technology, 2015) Alkaya, Naki; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologySalt is necessary for plants because of ion homeostasis; however, excess uptake of salt leads to damage in plant cells, which may even result in the death of the plant. Hordeum marinum, also known as sea barley, is a member of Poaceae family that resides in coastal areas, so it is thought that it may have a possible salt tolerance gene or genes. Therefore, this study aims to identify the genes involved in salt tolerance in Hordeum marinum by functional genomics method. After screening, seven transformant yeast colonies found and sequence analyses of these plasmids gave homology to hypothetical protein of Bipolaris oryzae. To confirm salt tolerance of this protein, salt sensitive yeast cell transfected by this candidate gene was checked in high salt concentration containing medium. Based on solid growth assay, these transgenic yeast cells could survive in 1M saline medium. Hence, it is hypothesized that Hordeum marinum and Bipolaris oryzae might have a symbiotic association. It is possible that in this association Bipolaris oryzae may play a role as endophytic fungus that might also confer salt tolerance in Hordeum marinum.Master Thesis Physiological and Genetic Characterization of Salt Tolerance in Tomato (lycopersicon Esculentum)(Izmir Institute of Technology, 2006) Göl, Deniz; Frary, Anne; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyPlant growth is limited by different environmental conditions. Salt stress is one of these conditions that affects plant growth. Elimination of salt from the soil is time consuming and very expensive. The most effective way to eliminate salinity effects is to produce salt tolerant crops. Both transgenic applications and molecular marker technology are of importance in producing salt tolerant plants. In this study, responses to salt stress of tomato were studied during the germination and vegetative stages of the life cycle. Inbred Backross Lines (IBLs) from a cross between salt-sensitive L. esculentum and a salt-tolerant L. pimpinellifolium were used for evaluation of salt tolerance during seed germination and QTL mapping. At the end of the germination study, it was observed that the IBLs have some degree of salt tolerance. L. esculentum alleles provided improved total percent germination on salt, however, L. pimpinellifolium alleles provided an improved rate of germination on salt. Thus, different parameters of salt tolerance are controlled by different mechanisms during seed germination. L. pennellii introgression lines (ILs) generated by crossing L. pennellii (LA716) to L. esculentum cv. M82 were used to map antioxidant traits related to salt tolerance at the vegetative stage of tomato. Plants of cultivated tomato (M82) and 32 IL lines were grown in aerated Hoagland solution in the greenhouse. At the seven-true leaf stage salt treatment was started and was achieved with the gradual addition of NaCl to the nutrient solution until 150 mM NaCl was reached. Superoxide dismutase (SOD) and catalase (CAT) activities were measured in the leaf tissues of these plants. Enzyme activities of the ILs were compared with M82 and QTLs associated with SOD and CAT activity under control and salt conditions were mapped.Master Thesis Identification of Salt Stress Responsive Protyeins in Wild Sugar Beet (beta Maritima) Using 2d-Page With Maldi-tof/Tof System(Izmir Institute of Technology, 2012) Çakıroğlu, Çiğdem; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyHigh salinity is one of the abiotic stresses, which affects the homeostasis, growth and productivity of plants. In plants, uptake of the non-essential salt ions negatively affects the anatomy, physiology and metabolism, changes the osmotic balance in cells and causes abundant dehydration. In this case, higher plants develop salt tolerance mechanisms such as induction of related signaling pathways, effluxion of salt ions, accumulation of these toxic ions in their vacuoles, activation of their detoxification mechanisms and production of osmoprotectans. In this study, identification of salt responsive proteins in moderately halophyte wild type sugar beet Beta vulgaris ssp. maritima was aimed. In order to investigate the protein-based natural stress tolerating mechanisms, plants were exposed to 150 mM NaCl and total proteins were extracted. Differentially expressed proteins were identified by proteomic approaches including MALDI-TOF/TOF mass spectrometry combined two dimensional polyacrylamide gel electrophoresis. The results revealed that enzymatic antioxidants and secondary members of antioxidative pathways are responsive in salt stress. In conclusion, these detected proteins demonstrate that antioxidative system may be the major defense mechanism in halophytic plants.