Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Crude Pectinolytic Enzymes Production in Fed-Batch Shake Flask Cultivation(01. Izmir Institute of Technology, 2023) Esen, Büşra Nur; Uzuner, Sibel; Uzuner, Sibel; Taşkent Sezgin, HümeyraThe use of waste in the production of enzymes, which is one of the products with high added value, is one of the right strategies to reduce the production cost of the product and sustainability movement. In this study, the production of polygalacturonase (PGase) and pectin lyase (PLase) enzymes from Bacillus subtilis ATCC 6633 in fed batch submerged fermentation, the conditions and composition of the fermentation medium and the effects of pretreatment methods (thermal, thermo-chemical, microwave assisted dilute acid (MW- DA)) on the conversion of fermentable sugar from black carrot pulp were investigated. The MW-DA was chosen the best with higher fermentable sugar content (FSC). The three different powers (300, 600, 850 W) and 3 different treatment time (30, 60, 90 s) were examined by Taguchi design. The highest FSC was found at 300 Watt for 30 seconds. MW-DA followed by ES produced the most fermentable sugar (0.493 g/g, 87.3% conversion). The amount of fermentable sugar was enhanced from 15.8% to 87.3% when MW-DA treatment is combined with enzymatic saccharification (ES). Yeast extract, whey and pea protein were examined as nitrogen sources. According to the enzyme activity results obtained, the fermentation medium was modified with pea protein. Certain concentrations (2.5%, 5%, 10%, 15%) were fed to the fermentation medium. The highest PGase activity was determined at the 15% feed concentration and 72th hours (164.34±2.26 U/L) whereas the highest PLase activity was obtained at 72th hours (188.22±1.72 U/L) at 5% feed concentration.Master Thesis Evaluation of Waste Management System in İzmir-Karaburun Peninsula From Sustainability Perspective(Izmir Institute of Technology, 2022) Hajatnia, Haniyeh; Ökten, Hatice EserGlobal solid waste generation is constantly rising, hence the need for management strategies that implement environmental improvements. The sustainable municipal solid waste management strategy for municipalities must include collection and transportation. The collection and transportation sector have been neglected while it is one of the most significant polluters. As a result, this study aims to model municipal solid waste transportation using Life Cycle Assessment (LCA) software which we used CCalC2 for this study and CML2001 methodology was used. To demonstrate how different approaches to waste management through transportation can reduce environmental impacts, LCA modeling was done for the three districts of Urla, Çeşme, and Karaburun, all of which are located on the Karaburun Peninsula. Each district was assigned three scenarios, with Scenario 0 representing current municipal practices, Scenario 1 representing a 50% reduction in plastic waste, and Scenario 2 representing a 50% reduction in all renewables. Results showed that only plastic separation might not be enough to achieve significant e reductions in environmental impacts. It has been demonstrated that in the transportation sector of Urla and Çeşme, Scenario 1 had a CO2 reduction of 3.7% and Karaburun had a CO2 reduction of 3.8% while Scenario 2 represented at least a 20% reduction of carbon footprint in all three districts. Findings of this research will support municipalities in the roadmaps they will choose for the Municipal Solid Waste Management applications.