Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Determination of Genetic Diversity and Antioxidant Content of the National Melon (cucumis Melo) Collection(Izmir Institute of Technology, 2008) Şığva, Hasan Özgür; Frary, Anne; Frary, AnneIn this study, characterization with 19 morphological criteria and two types of PCRbased molecular marker systems, Simple Sequence Repeats (SSRs, microsatellites) and Amplified Fragment Length Polymorphisims (AFLPs), were used to characterize genetic variability among 238 national melon (Cucumis melo L.) accessions and 12 different members of the Cucurbitaceae family.According to morphological analysis, all accessions were vigourous. The accessions showed a great variety of fruit shape, 53 (38.4%) accessions were globular (round), 51 (37.0%) were eliptical, 15 (10.9%) were ovate, 7 (5.1%) oblate, 2 (1.4%) elongate and one accession was flattened. Another agronomically important criterion was predominant fruit skin colour. A total of 55 (39.9%) accessions had light yellow predominant skin colour, 34 (24.6%) were pale green, 29 (21.0%) were orange, 9 (6.5%) were cream, 6 (4.4%) were green, 4 (2.9%) were blackish-green and 1 (0.7%) was dark green.In accordance with genetic characterization, a total of 345 polymorphic AFLP fragments (products of 10 AFLP primer combinations) and 93 SSR fragments (products of 12 SSR markers) were detected and used to calculate genetic distance using DICE matrix and UPGMA (Unweighted Pair Group Method) arithmetical averages. The average polymorphic AFLP fragments per combination was 34.5 and SSR fragments per marker was 7.75. The phylogenetic tree showed that groups were clearly separated by both marker systems. This study allowed the identification of the relationship between national melon accessions based on genetic similarity or differences.Forty three melon accessions were also analyzed for total water soluble antioxidant and total phenolic compound activities.Master Thesis Quantitative trait analysis in solanum lycopersicum x solanum peruvianum(Izmir Institute of Technology, 2009) Yüce, Duygu; Frary, AnneTomato is an important vegetable for both the economy and the human diet and it is a good model system for genetic studies. Because of tomato.s commercial importance, agronomic traits such as yield, fruit weight, size, color and firmness are very significant for the tomato processing industry and fresh consumption. However with increased attention on health, plant breeders also consider the improvement of health related traits of tomato such as antioxidant characters. Improvement of these desired traits is very difficult because many plant traits are controlled by more than one gene. In this study both health-related and agronomically important traits were characterized in an BC2F2 S. peruvianum mapping population of 118 individuals. All plants were phenotypically characterized for total water-soluble antioxidant activity, phenolic and vitamin C contents as well as several agronomic traits including fruit weight and shape, color and firmness. All antioxidant traits showed good variation in the population with the S. peruvianum parent having significantly higher values for all three antioxidant traits. Based on trait distributions and transgressive segregation in the population, it was expected that some alleles from the wild species S. peruvianum had the capacity for improvement of both antioxidant and agronomic traits of cultivated tomato. Both parents were genotypically characterized with 169 genetic markers including 96 COSII and 73 SSR markers. Good levels of polymorphism were identified with both types of marker. Thus, it was shown that the population contains sufficient trait and genotypic variation for efficient mapping of quantitative trait loci.