Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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COAR Access Rights: search.filters.coarAccessRights.embargoed accessSubject: search.filters.subject.Microfluidic device

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  • Master Thesis
    Cell Separation in Microfluidic Devices
    (01. Izmir Institute of Technology, 2022) Öksüz, Cemre; Tekin, Hüseyin Cumhur
    Cell separation is used to separate homogeneous and individual cell classes from a heterogeneous cell population. The efficiency and purity of these separated cells are of great importance in personalized medicine, regenerative medicine, disease monitoring and drug testing as well as in the therapeutic and diagnostic research. In this thesis, different microfluidic approaches were presented for cell separation. With this regard, a closed channel vacuum-integrated microfluidic chip was developed using an air permeability of a Polydimethylsiloxane and density-based separation of microparticles was performed. Besides, a centrifugal microfluidic system, Spinochip, was developed with one reservoir as inlet and outlet for the first time and different fluid manipulations were shown in the system. The system was applied to clinical tests of hematocrit measurements and white blood cell estimation using real patient samples. The developed system offered correlated results with clinical results. In addition to closed channel microfluidics, negative-magnetophoresis microfluidic chip was demonstrated for the size-based separation of microparticles and cells. In this regard, capturing rate of breast cancer cells (MCF-7) and human monocyte cells (U937) was investigated. The results showed that the approaches presented here could promote to the microfluidic studies for size-based cell separation.
  • Master Thesis
    Magnetic-Based Cell Manipulation in Microfluidic Devices
    (01. Izmir Institute of Technology, 2022) Özçelik, Özge Solmaz; Tekin, Hüseyin Cumhur
    Cell manipulation is the concept of altering cell movement. Different manipulation techniques have been demonstrated with microfluidic systems for various studies such as tissue engineering, circulating tumor cell (CTC) filtering, and other biomedical applications. For instance, cell patterning and filtering studies are being developed through different manipulation approaches in microfluidic platforms where one of these approaches is the magnetophoresis principle method. Positive and negative magnetophoresis can be utilized generally through labeling or non-labeling, respectively. In this thesis, two different cell manipulation platforms using negative magnetophoresis were developed for cell patterning and cell filtration applications. These platforms allow several advantages such as simple fabrication, easy control, and low cost. Compared to other devices, the developed microfluidic platforms do not require any labeling process for cells for magnetic manipulation. In the patterning platform, microparticle and cell patterns were formed inside a simple microfluidic channel with different tilted angles in <1.5 hours. Furthermore, in the filtration platform, large microparticles were separated from small microparticles with 98.25% trapping efficiency. Live/dead cell separation of human monocyte macrophage cells (U937) under different flow rates was also investigated. The suggested platforms could be useful for label-free magnetic cell patterning and filtering in biomedical applications.