Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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COAR Access Rights: search.filters.coarAccessRights.open accessSubject: search.filters.subject.Microfluidic device

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  • Master Thesis
    Design and Development of Paper-Based Microfluidics for Point-Of Applications
    (01. Izmir Institute of Technology, 2020) Özefe, Fatih; Arslan Yıldız, Ahu; Yıldız, Ümit Hakan
    Paper-based microfluidics is a subarea of microfluidics which is recently used in various applications from diagnostics to environmental monitoring, and to food safety. In such microfluidic systems, a test platform is formed from a paper substrate instead of silicon and polymers, such as poly-dimethylsiloxane, poly-methyl methacrylate, and etc. The main goal of this thesis is the development and fabrication of a paper-based microfluidic device (μPAD), which could be used in point-of-care (POC) applications. The characterizations of μPADs, which were fabricated via laser ablation methodology, were performed in terms of their surface and barrier characteristics, and liquid sample flows within μPADs. Depending on the characterization, nine different fabrication parameters, 10P40S (10%Power & 40%Speed), 10P60S, 20P90S, 30P50S, 30P100S, 40P80S, 40P100S, 70P80S, and 70P100S, were identified as optimized fabrication parameters. Also, two designed models of μPADs, 1S4T-Type2 and 1S4T-Type3, were selected to be used in the detection of BSA and recombinant Hepatitis C Virus (HCV) protein. The BSA and HCV (1 mg/ml) in PBS solution were successfully detected via naked eye depending on the colorimetric sensing through micro-paper enzyme linked immunosorbent assay (μP-ELISA) protocol. Moreover, the limit of detection (LoD) values for HCV were determined in 1S4T-Type2 μPAD as 1.000, 0.883, and 0.796 ng/ml when the detection was performed via naked eye, smart-phone, and bright-field microscope, respectively. Also, the easily-disposable 1S4T-Type2 μPAD provided 14 times faster and 45 times cheaper detection of HCV compared to conventional ELISA techniques. Consequently, the developed 1S4T-Type2 μPAD presented low-cost, easy-to-use, and rapid detection of HCV as POC devices.
  • Master Thesis
    Design and Fabrication of Microfluidic Device That Allows Investigation of Distance Dependent Interactions of Two Different Cell Types
    (Izmir Institute of Technology, 2014) Sağlam, Murat; Pesen Okvur, Devrim; Özyüzer, Lütfi
    The main studies of in this thesis, the mold and a microfluidic device are achieved by using SU-8 photoresist and PDMS polymer. Firstly, molds are obtained which are thickness ranging from 30 to 400 μm by using SU-8 photoresist with UV lithography technique and this molding will use for shaping polydimethylsiloxane (PDMS) polymer. Finally, PDMS molds combined with the glass surface to create a three dimensional reservoirs. Microfluidic device that allows investigation of distance dependent interactions, two factors are positioned at certain distances from each other and the microfluidic device is allowed to investigation of distance dependent interaction of two factors. There is an alternating width channel between two channels which have each of two factors. These three channels are separated from each other by colonnades, not by walls, therefore physical, chemical and biological interactions are possible between the factors. Necessary physical, chemical, and biological conditioning can be provided by the reservoirs which are neighbor of channels including factors. Microfluidic chip has a lot of advantages that are small liquid volumes (pL-μL), precise spatial & temporal control, successfully mimic the physiological context, highthroughput analysis, low fabrication costs; portable and safer therefore it facilitates us to refine our methods of analysis and development in cell biology investigations and determining the content of chemical samples.