Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Department: search.filters.department.Thesis (Master)--İzmir Institute of Technology, Molecular Biology and GeneticsPublisher: search.filters.publisher.İzmir Institute of Technology

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Now showing 1 - 3 of 3
  • Master Thesis
    Hiv-1 Regulatory Gene Dependent Expression of a Toxic Gene
    (İzmir Institute of Technology, 2006) Yeğin, Zeynep; Arslanoğlu, Alper
    From the first day it was discovered, HIV remains as one the major health threats of 21st century and the methods tried up to now focused on the short-term solutions which were efficient at blocking HIV replication, but also resulted with drugresistant strains, instead of methods which would completely eliminate HIV-infected cells from potential reservoirs. In this study, the design of a special DNA vector encoding a toxic protein to be expressed only in cells infected with HIV, thereby not damaging to healthy cells and the test of the efficacy of this vector in the cell culture conditions without using HIV infection was aimed. The toxic gene (suicide gene) presumed to create the desired effect was placed under the transcriptional control of HIV promoter LTR and so that the expression of the toxic gene was made dependent upon the tat regulator gene of HIV. In order to prevent leaky gene expression stemming from the basal gene expression from LTR even if it was not induced by Tat, and thereby having potential to damage healthy cells, the prerequisite cis-acting DNA sequences were cloned downstream of the toxic gene. So that, the transcripts produced could retain in the nucleus and would require the function of a second regulator protein 'Rev' which is a molecular chaperone for being transmitted into the cytoplasm. If the efficiency of this model prooved, a full-protective suicide vector will have been designed and this vector may be suggested to be tested in gene therapy trials of HIV infection in the future.
  • Master Thesis
    Characterization and Genetic Mapping of Health Related Traits in Tomato
    (İzmir Institute of Technology, 2005) Rusçuklu, Dane; Doğanlar, Sami
    Tomato is consumed in the daily diet and has a diverse nutritional content including antioxidants, lycopene, vitamin C and phenolics. However, the content of these compounds in tomato cultivars could be higher. There are two ways of increasing the nutritional quality of tomato. One of them is a transgenic way which is still questioned and is not approved in many countries. The other way is molecular breeding which is based on introgression of wild genes into cultivated tomatoes via the combination of classical breeding with molecular marker analysis. This method lets us obtain nontransgenic tomatoes with increased nutritional quality. In this project a population that was obtained from a cross between wild type L.pimpinellifolium and cultivated tomato L.esculentum was used. This population includes 145 inbred backcross lines (IBLs). It was expected that some individuals of this population had increased nutritional quality derived from the wild parent. In order to test this hypothesis, antioxidant activity of each line was measured. The method used to measure antioxidant activity was based on spectrophotometric measurement which gave mol Trolox equivalent antioxidant activity. It was observed that the IBLs were phenotypically close to L.esculentum and that some individuals did have higher antioxidant activity than the cultivated parent. The population was also mapped with molecular markers and 31 possible QTLs which control antioxidants in tomato were found. The source of alleles associated with increased antioxidant characters was usually L.pimpinellifolium. This result confirmed that wild type tomatoes can be a resource for increasing nutritional quality of tomatoes.
  • Master Thesis
    Isolation of Haloalkaliphilic Microorganisms From Leather Industry
    (İzmir Institute of Technology, 2004) Çoban, Hatice Sevgi; Yenidünya, A.Fazil
    Haloalkalophiles are extremophilic microorganisms that are adapted to saline and alkaline conditions. Different species of them have been isolated so far from soda lakes and soil samples. Haloalkalophilic microorganisms have significant adaptive mechanisms to avoid denaturing effect of salts and to balance their interior pH. Extracellular enzymes that are produced by these halophilic and alkalophilic microorganism are applicable for industrial purposes. Therefore isolation of these organisms from their habitats and study on genotypic characterization constitute initial steps for furter biotechnological studies. In this study, processing steps of leather factories and their wastewater were chosen for sampling. In order to isolate target microorganisms Horikoshi-I medium including 12% NaCl was used. After isolation microorganisms were purified. Phenotypic tests were applied (Gram staining, catalase and oxidase behaviors, spore observation by phase contrast microscope, sensitivity to antibiotics and extracellular enzyme screenings). For genotypic characterization, genomic DNA was isolated and 16S-ITS rDNA region was amplified. Differentiation was achieved according to Restriction Fragment Length Polymorphism (RFLP) method by using Hae III and Taq I endonucleases. Isolates, which represented the different RFLP patterns, were chosen for building up the haplotype groups. As a result of the study seven different RFLP haplotypes were identified.Moreover, 16S ribosomal DNA partial sequencing was also performed on some of the strains in. These haloalkalophilic microorganisms and their enzymes could be used in different biotechnological studies in the future for various industrial applications.