Master Degree / Yüksek Lisans Tezleri

Permanent URI for this collectionhttps://hdl.handle.net/11147/3008

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Publisher: search.filters.publisher.01. Izmir Institute of TechnologySubject: search.filters.subject.Microfluidics

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  • Master Thesis
    Development of Microfluidic Devices for Investigating Small Molecule Induced Chemotaxis of Dendritic Cells
    (01. Izmir Institute of Technology, 2023) Khurram, Muhammad Maaz; Bedir, Erdal; Tekin, Hüseyin Cumhur
    Microfluidics is the core branch of science and technology in which interdisciplinary research is conducted with a low amount of samples in microchannels ranging from 10-100 μm. The main objective of this thesis is to design and fabricate a chemotaxis microfluidic device (CMD) from the poly-methyl methacrylate (PMMA) substrate to analyze the immune cell behavior against cancer cells. The patterns of the three-layered CMD were generated using laser ablation. During the fabrication, Power (P) and Speed (S) values were varied to determine the optimal P-S combination. Then, the structural properties of microfluidic channels in the CMD were examined via microscope. The mechanical properties and liquid handling abilities of CMDs were also investigated through tensile and leakage tests, respectively. Moreover, cell viability of DC2.4 dendritic cells (DCs) and B16-F10 murine melanoma (B16-F10) cells in CMDs sterilized through either autoclaving or UV treatment were determined to test the suitability of CMDs via Live/Dead Assay. The highest cell viability for DCs and B16-F10 was obtained in autoclaved CMDs. For the maturation of DCs before seeding into CMD, DCs were stimulated with lipopolysaccharide (LPS) and Astragaloside VII (AST-VII) at various concentrations. While the cytotoxicity of LPS and AST-VII were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, the expression levels of specific chemokine receptors were also analyzed through flow cytometry. Lastly, stimulated DCs and B16-F10 were simultaneously cultured in the CMD, and the migratory behavior of DCs against B16-F10 was time-dependently studied. Consequently, CMD that provided cost-effective and rapid analysis of intercellular interactions was successfully developed.
  • Master Thesis
    Design and Development of Paper-Based Microfluidics for Point-Of Applications
    (01. Izmir Institute of Technology, 2020) Özefe, Fatih; Arslan Yıldız, Ahu; Yıldız, Ümit Hakan
    Paper-based microfluidics is a subarea of microfluidics which is recently used in various applications from diagnostics to environmental monitoring, and to food safety. In such microfluidic systems, a test platform is formed from a paper substrate instead of silicon and polymers, such as poly-dimethylsiloxane, poly-methyl methacrylate, and etc. The main goal of this thesis is the development and fabrication of a paper-based microfluidic device (μPAD), which could be used in point-of-care (POC) applications. The characterizations of μPADs, which were fabricated via laser ablation methodology, were performed in terms of their surface and barrier characteristics, and liquid sample flows within μPADs. Depending on the characterization, nine different fabrication parameters, 10P40S (10%Power & 40%Speed), 10P60S, 20P90S, 30P50S, 30P100S, 40P80S, 40P100S, 70P80S, and 70P100S, were identified as optimized fabrication parameters. Also, two designed models of μPADs, 1S4T-Type2 and 1S4T-Type3, were selected to be used in the detection of BSA and recombinant Hepatitis C Virus (HCV) protein. The BSA and HCV (1 mg/ml) in PBS solution were successfully detected via naked eye depending on the colorimetric sensing through micro-paper enzyme linked immunosorbent assay (μP-ELISA) protocol. Moreover, the limit of detection (LoD) values for HCV were determined in 1S4T-Type2 μPAD as 1.000, 0.883, and 0.796 ng/ml when the detection was performed via naked eye, smart-phone, and bright-field microscope, respectively. Also, the easily-disposable 1S4T-Type2 μPAD provided 14 times faster and 45 times cheaper detection of HCV compared to conventional ELISA techniques. Consequently, the developed 1S4T-Type2 μPAD presented low-cost, easy-to-use, and rapid detection of HCV as POC devices.