WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7150

Browse

Filters

2014 - 20203

Settings

Access Right: Open AccessSubject: search.filters.subject.Colon cancer

Search Results

Now showing 1 - 3 of 3
  • Article
    Citation - WoS: 1
    İnsan Kolon Kanseri Hücrelerine Karşı İnorganik Nanopartikül-temelli İlaç Taşıyıcı Sistemlerin Kullanılması: Partikül Büyüklüğünün Antikanser Aktivitesine Etkisi
    (Gazi Üniversitesi, 2020) Dağlıoğlu, Cenk; Dağlıoğlu, Cenk; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Today's nanoparticle technology enables the synthesis of nanoparticle-based drug delivery systems with desired size, shape, and materials especially for the applications of cancer nanomedicine. Thereby, understanding impact of particle sizes on anticancer activity will contribute to development of new drug delivery systems in cancer therapy. For this reason, in this study, two different sized nanoparticles (with -55 and 314 nm) were used as drug delivery systems and the effects of their size on the cellular uptake, cytotoxicity and apoptosis were investigated against the human colon carcinoma Caco-2 and HCT-116 cells. The results demonstrated that small nanoparticles promoted fast nanoparticle accumulation in both cancer cells in comparison to large particles. Small nanoparticles exhibited higher cytotoxicity in cancer cells with lower half maximal inhibitory concentration (IC50) values than large nanoparticles in 48 h. On the other hand, both nanoparticles showed similar IC50 values after 72 h prolonged exposure. Moreover, it was found that small nanoparticles increased the number of apoptotic cells in 24 h, whereas large nanoparticles induced apoptosis when exposure time increased to 72 h. These observations show that small sized drug delivery systems could be more efficient for improving the anticancer effects of chemotherapeutic drugs against human colon carcinoma as compared to large sized drug delivery systems.
  • Article
    Citation - WoS: 9
    Citation - Scopus: 10
    An Answer To Colon Cancer Treatment by Mesenchymal Stem Cell Originated From Adipose Tissue
    (Mashhad University of Medical Sciences, 2018) İplik, Elif Sinem; Baran, Yusuf; Kozanoğlu, İlknur; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.
  • Article
    Citation - WoS: 33
    Citation - Scopus: 37
    Apoptotic Effects of Quercitrin on Dld-1 Colon Cancer Cell Line
    (Springer Verlag, 2014) Çinçin, Zeynep Birsu; Ünlü, Miray; Baran, Yusuf; Ünlü, Miray; Baran, Yusuf; Çakmakoğlu, Bedia; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Quercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.