WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Conference Object Accumulation of Apoptotic Ceramides Increased Apoptotic Effects of Nilotinib in Philadelphia Positive Meg-01 Cells Synergistically(Ferrata Storti Foundation, 2009) Baran, Yusuf; Baran, Yusuf; Gençer, Emel Başak; Ural, Ali Uğur; Avcu, Ferit; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology[No abstract available]Conference Object Increasing De Novo Synthesis of Ceramides Increased Nilotinib Induced Apoptosis in Human Meg-01 Chronic Megacaryoblastic Cells(Ferrata Storti Foundation, 2009) Baran, Yusuf; Baran, Yusuf; Gençer, Emel Başak; Ural, Ali Uğur; Avcu, Ferit; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology[No abstract available]Conference Object Targeting Ceramide Metabolism Increased Sensitivity of Philadelphia Positif Chronic Megacaryoblastic Leukemia Cells To Dasatinib(Ferrata Storti Foundation, 2009) Gençer, Emel Başak; Baran, Yusuf; Ural, Ali Uğur; Avcu, Ferit; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology[No abstract available]Article Citation - WoS: 16Citation - Scopus: 18Roles of Ceramide Synthase and Ceramide Clearence Genes in Nilotinib-Induced Cell Death in Chronic Myeloidleukemia Cells(Informa Healthcare, 2011) Camgöz, Aylin; Gençer, Emel Başak; Baran, Yusuf; Avcu, Ferit; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyIn this study, we aimed to increase the sensitivity of human K562 and Meg-01 chronic myeloid leukemia (CML) cells to nilotinib by targeting bioactive sphingolipids, in addition to investigating the roles of ceramide metabolizing genes in nilotinib induced apoptosis. Cytotoxic effects of nilotinib, C8:ceramide, glucosyle ceramide synthase (GCS) and sphingosine kinase-1 (SK-1) inhibitors were determined by XTT cell proliferation assay and synergism between the agents was determined by isobologram analysis. Also, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) results demonstrated that expression levels of longevity assurance (LASS) genes in response to nilotinib were correlated with sensitivity to nilotinib. For the first time, The results of this study showed for the first time that nilotinib induces apoptosis through upregulating ceramide synthase genes and downregulating SK-1 in CML cells in addition to inhibition of BCR/ABL. On the other hand, manipulating bioactive sphingolipids toward generation/accumulation of ceramides increased the apoptotic effects of nilotinib in CML cells.