WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
Browse
4 results
Search Results
Conference Object Citation - WoS: 3Cadmium-Induced Physiological and Genotoxic Alterations in Bryophyllum Daigremontianum(Global Nest, 2013) Özyiğit, İbrahim İlker; Yılmaz, Seçil; Doğan, İlhan; Sakcalı, Mehmet Serdal; Kekeç, Güzin; Demir, Göksel; Severoğlu, ZekiWhen evaluating the negative impacts of heavy metals in the environment, plants can be used as biological indicators in assessing damage done by bio-accumulation of heavy metals. For detecting the genotoxic effects of heavy metals, the RAPD-PCR technique is applied in plants. In this study, Blyophyllum daigremontianum was used as a bio-indicator for Cadmium (Cd) pollution. B. daigremontianum plantlets were obtained from bulbiliferous spurs. They were grown singly in standard pots and each of the experimental groups of 5 replicates were watered daily with Hoagland solution (20 ml) containing 0, 50, 100, 200 and 400 mu M Cd for two months. Plantlets were harvested at the end of the two-month experimental period and photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll, chlorophyll a/b and carotenoids) contents of B. daigremontianum in response to Cd stress were investigated. The comparison between unexposed and exposed B. daigremontianum groups revealed gradual reductions of photosynthetic pigment contents (-40.57% for chlorophyll a, -37.63% for chlorophyll b, -36.27% for total chlorophyll, -20.58% for chlorophyll a/b and -37.66% for carotenoids) at 400 pM and a genomic instability as Cd concentration increases. The results indicated that changes in RAPD-PCR profiles following Cd treatments included a variation in band intensities, a loss of normal bands, and the appearance of new bands compared to unexposed plantlets. Cd concentrations of the collected samples were measured by employing ICP-OES during the study. The concentrations of Cd at 400 mu M Cd treatment were increased significantly in leaves (-5.03 fold), stems (-6.28 fold) and roots (-3.90 fold) of B. daigremontianum. The present study evidences that as an investigation tool for environmental toxicology, the RAPD technique can be useful and can be applied as a suitable biomarker assay for the detection of genotoxic effects of Cd pollution on plants.Article Citation - WoS: 6Citation - Scopus: 9Detection of Physiological and Genotoxic Damages Reflecting Toxicity in Kalanchoe Clones(Global Network Environmental Science & Technology, 2016) Özyiğit, İbrahim İlker; Yılmaz, S.; Doğan, İlhan; Sakçalı, Mehmet Serdal; Tombuloğlu, Güzin; Demir, GökselIn order to make assessments in understanding of physiological and genotoxic effects of imposing cadmium (Cd) on photosynthetic pigment contents along with the changes occurring in genetic material of Kalanchoe plants were used in relation to various Cd-treatments. Young plantlets were originated from a single host plant as clones, and developed in vitro. Developed clones were grown in standard pots with daily watering of Hoagland solution (20 ml) containing different concentrations of cadmium chloride for two months. Cd concentrations of the collected samples were measured by employing ICP-OES and RAPD-PCR technique was applied for detecting the genotoxic effects of Cd. After two month of experimental period, the comparisons between unexposed and exposed Kalanchoe clone groups revealed reductions in photosynthetic pigment contents, especially at the highest level of Cd exposure and a genomic instability when application of Cd concentration increases. RAPD-PCR analyses demonstrated the distinguishable banding pattern in number and band intensities between Cd-treated and control groups. In addition, progressive Cd accumulations in leaves, stems and roots of plant samples were observed when the application of exposure level increased.Article Citation - WoS: 16Citation - Scopus: 16Screening of Damage Induced by Lead (pb) in Rye (secale Cereale L.) – a Genetic and Physiological Approach(Taylor and Francis Ltd., 2016) Özyigit, İbrahim İlker; Doğan, İlhan; İğdelioğlu, Sezen; Filiz, Ertuğrul; Karadeniz, Sedat; Uzunova, ZeynepThe fields in which lead (Pb) finds application in the modern world have increased dramatically in recent years. As a consequence of this intensive utilization of Pb, its toxicity tends to pose more and more environmental problems. The aim of this study was to evaluate the genotoxic potential of Pb and to characterize some physiological parameters in Secale cereale under Pb stress. Plants were subjected to different exposure levels of Pb (0, 100, 200 and 400 µmol/L) for two weeks. At the end of the experimental period, the effects of Pb exposure on the photosynthetic pigments content (chlorophyll a and b, total chlorophyll, chlorophyll a/b and carotenoids) and genetic material of S. cereale were studied. To evaluate the genotoxic effect of Pb, random amplified polymorphic DNA – polymerase chain reaction (RAPD-PCR) was employed. The obtained results showed alteration in the photosynthetic pigments content and RAPD-PCR profiles of S. cereale grown in the presence of Pb. The alterations in the RAPD-PCR profiles following Pb treatments appeared to be losses of normal bands and occurrences of new bands compared to unexposed plantlets. Overall, the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids decreased by 6.68%, 6.08%, 2.89% and 8.57%, respectively, under severe Pb stress (400 µmol/L).Article Citation - WoS: 18Citation - Scopus: 20Assessment of Cd-Induced Genotoxic Damage in Urtica Pilulifera L. Using Rapd-Pcr Analysis(Taylor and Francis Ltd., 2016) Doğan, İlhan; Özyiğit, İbrahim İlker; Tombuloğlu, Güzin; Sakçalı, Mehmet Serdal; Tombuloğlu, HüseyinPlants can be used as biological indicators in assessing the damage done by bioaccumulation of heavy metals and their negative impact on the environment. In the present research, Roman nettle (Urtica pilulifera L.) was employed as a bioindicator for cadmium (Cd) pollution. The comparisons between unexposed and exposed plant samples revealed inhibition of the root growth (∼25.96% and ∼45.92% after treatment with 100 and 200 µmol/L Cd concentrations, respectively), reduction in the total soluble protein quantities (∼53.92% and ∼66.29% after treatment with 100 and 200 µmol/L Cd concentrations, respectively) and a gradual genomic instability when the Cd concentrations were increased. The results indicated that alterations in randomly amplified polymorphic DNA (RAPD) profiles, following the Cd treatments, included normal band losses and emergence of new bands, when compared to the controls. Also, the obtained data from F1 plants, utilized for analysis of genotoxicity, revealed that DNA alterations, occurring in parent plants due to Cd pollution, were transmitted to the next generation.