WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7150

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Author: search.filters.author.Doğanlar, SamiSubject: search.filters.subject.Olea europaea

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  • Article
    Citation - WoS: 17
    Citation - Scopus: 19
    Cultivar Origin and Admixture Detection in Turkish Olive Oils by Snp-Based Caps Assays
    (American Chemical Society, 2015) Uncu, Ali Tevfik; Frary, Anne; Frary, Anne; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    The aim of this study was to establish a DNA-based identification key to ascertain the cultivar origin of Turkish monovarietal olive oils. To reach this aim, we sequenced short fragments from five olive genes for SNP (single nucleotide polymorphism) identification and developed CAPS (cleaved amplified polymorphic DNA) assays for SNPs that alter restriction enzyme recognition motifs. When applied on the oils of 17 olive cultivars, a maximum of five CAPS assays were necessary to discriminate the varietal origin of the samples. We also tested the efficiency and limit of our approach for detecting olive oil admixtures. As a result of the analysis, we were able to detect admixing down to a limit of 20%. The SNP-based CAPS assays developed in this work can be used for testing and verification of the authenticity of Turkish monovarietal olive oils, for olive tree certification, and in germplasm characterization and preservation studies.
  • Article
    Citation - WoS: 27
    Citation - Scopus: 28
    Genetic Diversity of Turkish Olive Varieties Assessed by Simple Sequence Repeat and Sequence-Related Amplified Polymorphism Markers
    (Crop Science Society of America, 2011) Işık, Neslihan; Doğanlar, Sami; Frary, Anne; Doğanlar, Sami; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Olive (Olea europaea L.) is one of the most important and characteristic fruit crops of the Mediterranean region. This crop has been grown in Turkey for more than 3000 yr and many varieties are currently grown in five geographic regions. In this study, the genetic diversity of 66 olive varieties from these regions was assessed using simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) markers. The 13 SSR markers yielded 89 alleles with an average polymorphism information content (PIC) of 0.29 and 6.8 alleles per marker while the 13 SRAP primer combinations generated 103 polymorphic alleles with an average PIC of 0.24 and 7.9 alleles per combination. Although SSR markers revealed higher levels of polymorphism than SRAP markers, both systems revealed considerable molecular genetic diversity in Turkish olive varieties. For both marker types, clustering analysis using the Dice similarity coefficient and the unweighted pair group method with arithmetic means (UPGMA) produced dendrograms with similar clustering and some region-specific grouping of varieties. Overall, Southeast Anatolian and Marmara varieties were found to be more genetically different than those from the other regions (Aegean, Mediterranean, and Black Sea) and homonymous varieties were identified: three pairs of varieties ('Egriburun', 'Celebi', and 'Tasarasi') had the same names but were genetically distinct. © Crop Science Society of America.