WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Article Citation - WoS: 15Citation - Scopus: 16The Usability of Juniperus Virginiana L. as a Biomonitor of Heavy Metal Pollution in Bishkek City, Kyrgyzstan(Taylor & Francis, 2015) Kurmanbekova, Gülbübü; Doğan, İlhan; Severoğlu, Zeki; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim Ertuğrul; Kaşoğlu, Gülten; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyUncontrolled and unplanned urbanization and industrialization due to increase of population and rapid industrial development have created severe environmental problems in Kyrgyzstan during the last few decades. In this study, Juniperus virginiana, a dioecious species, was employed in order to make assessment of the heavy metal pollution rate in the area and of the heavy metal pollution impact on the mineral nutrient status of the plant. For this study, leaf (washed and unwashed) and bark samples of J. virginiana, and its co-located soil samples were collected from eight different stations, all in the capital of Kyrgyzstan, Bishkek, in 2012 vegetation period. The standard procedures were used and the determinations of heavy metal and nutrient element contents (Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Pb and Zn) in all samples were done using inductively coupled plasma-optical emission spectroscopy. According to our measurements, J. virginiana was found to be capable of accumulating a considerable amount of metals and the mineral nutrient uptake pattern was altered because of metal deposition in the plant, which showed a contamination risk in the area.Conference Object Citation - WoS: 3Cadmium-Induced Physiological and Genotoxic Alterations in Bryophyllum Daigremontianum(Global Nest, 2013) Özyiğit, İbrahim İlker; Doğan, İlhan; Yılmaz, Seçil; Doğan, İlhan; Sakcalı, Mehmet Serdal; Kekeç, Güzin; Demir, Göksel; Severoğlu, Zeki; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyWhen evaluating the negative impacts of heavy metals in the environment, plants can be used as biological indicators in assessing damage done by bio-accumulation of heavy metals. For detecting the genotoxic effects of heavy metals, the RAPD-PCR technique is applied in plants. In this study, Blyophyllum daigremontianum was used as a bio-indicator for Cadmium (Cd) pollution. B. daigremontianum plantlets were obtained from bulbiliferous spurs. They were grown singly in standard pots and each of the experimental groups of 5 replicates were watered daily with Hoagland solution (20 ml) containing 0, 50, 100, 200 and 400 mu M Cd for two months. Plantlets were harvested at the end of the two-month experimental period and photosynthetic pigment (chlorophyll a, chlorophyll b, total chlorophyll, chlorophyll a/b and carotenoids) contents of B. daigremontianum in response to Cd stress were investigated. The comparison between unexposed and exposed B. daigremontianum groups revealed gradual reductions of photosynthetic pigment contents (-40.57% for chlorophyll a, -37.63% for chlorophyll b, -36.27% for total chlorophyll, -20.58% for chlorophyll a/b and -37.66% for carotenoids) at 400 pM and a genomic instability as Cd concentration increases. The results indicated that changes in RAPD-PCR profiles following Cd treatments included a variation in band intensities, a loss of normal bands, and the appearance of new bands compared to unexposed plantlets. Cd concentrations of the collected samples were measured by employing ICP-OES during the study. The concentrations of Cd at 400 mu M Cd treatment were increased significantly in leaves (-5.03 fold), stems (-6.28 fold) and roots (-3.90 fold) of B. daigremontianum. The present study evidences that as an investigation tool for environmental toxicology, the RAPD technique can be useful and can be applied as a suitable biomarker assay for the detection of genotoxic effects of Cd pollution on plants.Article Evaluation of Aluminum Stress Induced Antibacterial and Antifungal Activities in Roman Nettle(Parlar Scientific Publications, 2019) Özyiğit, İbrahim İlker; Doğan, İlhan; Doğan, İlhan; Yalçın, İbrahim Ertuğrul; Severoğlu, Zeki; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyIn this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia coli, E. coli O157:H7 and Bacillus cereus) and fungus (Penicillum sp., Saccharomyces cerevisiae, Candida tropicans and C. albicans) by using the disc diffusion and agar well methods. The accumulated Al was measured by using ICP-OES in the leaves of studied plant samples. Additionally, a control group (water + 11.31 mg l(-1) Al) was prepared and applied to selected bacteria and fungi in order to understand the reason for obtained antimicrobial activities of Roman nettle is whether because of the compounds isolated from nettle leaves exposed to Al stress, or Al itself accumulated in leaves. The data proved that inhibitory antimicrobial effects were altered in U pilulifera upon the application of Al stress, especially on fungi species.