WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Conference Object Potential Application of Natural Phenolic Antimicrobials and Edible Film Technology Against Bacterial Plant Pathogens(International Society for Horticultural Science, 2020) Alkan, Derya; Yemenicioğlu, AhmetIn this study, antimicrobial edible film technology used frequently for inhibition of human pathogens in processed food was employed as a postharvest bio-based preservation method to prevent bacterial spoilage of fresh fruits and vegetables. Antimicrobial edible zein films were developed by incorporation of pure phenolic acids (PAs) such as gallic acid (GA), cinnamic acid (CA), vanillic acid (VA); essential oils (EOs) such as carvacrol (CAR), thymol (THY), eugenol (EUG) and citral (CIT) and phenolic extract (PE) from clove. In order to test synergetic effect of PAs, different amounts of PAs were added into film solution to obtain films containing mixture of GA and CA, GA and VA, CA and VA at different concentrations. The antimicrobial performances of edible films were tested on selected plant pathogenic bacteria including Pseudomonas syringae, Erwinia amylovora, Xanthomonas vesicatoria and Erwinia carotovora. The overall results of antimicrobial tests showed that films containing PAs between 1 and 4 mg cm-2 inhibited all pathogens while EOs at concentrations between 2 and 4 mg cm-2 and clove extract between 4 and 8 mg cm-2 were found effective against pathogens except P. syringae. Morover, the incorporation of combination of different PA showed greater inhibitory activity on plant pathogens than incorporation of a single PA into films. The edible zein coatings have already been applied for coating of fresh fruits and vegetables at the postharvest stage to suppress their respiration rate and prolong their refrigerated storage. This work clearly showed the benefit of incorporating a single or mixture of antimicrobial phenolic compounds, EOs and PEs in edible zein films against bacterial plant pathogens. The developed films might be employed against plant pathogens by coating fresh fruits and vegetables, seeds, seedlings, tree stems, soil as well as agricultural tools and constructions without using toxic chemicals. © 2020 International Society for Horticultural Science. All rights reserved.Article Citation - WoS: 64Citation - Scopus: 77Development of Flexible Antimicrobial Zein Coatings With Essential Oils for the Inhibition of Critical Pathogens on the Surface of Whole Fruits: Test of Coatings on Inoculated Melons(Elsevier, 2019) Boyacı, Derya; Iorio, Gianmarco; Sözbilen, Gözde Seval; Alkan, Derya; Trabattoni, Silvia; Pucillo, Flavia; Yemenicioğlu, AhmetThis study aimed to develop essential oil (EO)-containing antimicrobial coatings for the inhibition of pathogenic bacteria contamination on fruit peels. Incorporation of eugenol (EUG), carvacrol (CAR), and thymol (THY) into films at > 1% (w/w) eliminated the typical brittleness of zein films. However, EUG outperformed CAR and THY in terms of mechanical properties. Films with >= 2% (w/w) CAR and THY and >= 3% EUG showed clear zones against L. innocua and E. coli in agar medium at 37 degrees C. All EO-containing films also inhibited L. innocua and E. coli inoculated at their surfaces by minimum 3.9 and 2.7 decimal (D) within 1 day at 10 degrees C. Moreover, 2% EUG-containing zein coatings caused 2-3 decimal reduction in L. innocua and E. coli counts of inoculated melon surfaces at 10 degrees C. Unlike the bare zein coatings, flexible EUG-containing films on melons did not show cracking or detachment. Zein films loaded with EUG showed a highly hydrophilic surface. The best oxygen barrier performance was observed for the EUG-richest formulation (i.e., EUG at 3%), and this was attributed to a homogenizing effect of the EO that eventually led to a denser and hole-free network. This work suggested that flexible coatings of zein containing EOs could inhibit pathogens embedded in the rough peel surface of melons.Conference Object Citation - WoS: 4Citation - Scopus: 4Antioxidative and Antimicrobial Screening of 19 Commercial Essential Oils in Turkey(International Society for Horticultural Science, 2009) Bulut, Çisem; Altıok, Evren; Bayraktar, Oğuz; Ülkü, SemraPlant oils have been used for wide variety purposes for many years in Turkey. These essential oils have usage in many areas such as perfumery, cosmetic, food flavoring and folkloric medicine. In the study, 19 commercial essential oils were studied by means of antibacterial and antioxidant capacity. Antibacterial test was performed against S. aureus, S. epidermidis, K. pneumoniae and E. coli using standard disc diffusion method. Penicillin, gentamicin and vancomycin discs were also included. Inhibition zones (mm) were recorded to indicate antibacterial property. Penicillin (44 mm) and vancomycin (14 mm) could inhibit only S. aureus. Except essential oils of Juniperus nana (Ardiç), Ocimum basilicum (Feslegen), Urtica dioica (Isirgan), Foeniculum vulgare (Rezene) ressential oils indicated antimicrobial activity against some of the test microorganims. The most active essential oil was found as Thymus serpyllum (Kekik). It indicated highest inhibition zones for other microorganisms among others. Antioxidant capacities were measured using photochemiluminescence (PCL) method. The highest antioxidant activity was measured for Dianthus caryophyllus (Karanfil) as 547.781 103 ug trolox/mL of oil. It was followed by Thymus serpyllum (Kekik), Lilium candidum (Zambak) and Matricaria chamomilla (Papatya). Especially, Thymus serpyllum and Dianthus caryophyllus have significant antimicrobial and antioxidant capacity therefore it could be suggested for further investigations and applications.