WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Article Developing Gold Nanoparticles Decorated With Carbon-Dots for Multiplexed Cellular Imaging(IOP Publishing Ltd, 2025) Özçelik, Serdar; Saydullaeva, Iroda; Ozcelik, Serdar; 01. Izmir Institute of Technology; 04. Faculty of Science; 04.01. Department of ChemistryThis study focuses on developing a novel hybrid nanomaterial composed of gold nanoparticle decorated with carbon dots, termed AuNP@C-dot, as a versatile platform for multiplexed imaging. Structural and spectral characterizations confirmed the successful conjugation of C-dots to AuNPs via covalent bonding, as evidenced by FTIR, X-ray photoelectron spectra, HRTEM analyses, and UV-Vis and fluorescence spectroscopies. The fluorescence intensities of C-dots are doubled through the conjugation to the AuNPs. The conjugation of fluorescent C-dots to plasmon-resonant AuNPs enables simultaneous multicellular imaging by taking advantage of the fluorescent signaling of C-dots and the scattering signaling of AuNPs. In vitro studies using human lung cell lines (A549 and BEAS-2B) confirmed the multiplexed imaging and revealed efficient cellular uptake and subcellular localization of AuNP@C-dots, including nuclear translocation, which is critical for radiotherapy and photodynamic therapy. Cell viability assessments utilizing a colorimetric assay for measuring cell metabolic activity and a colony formation assay demonstrated good biocompatibility of AuNP@C-dots at relevant concentrations. It can be envisioned that the AuNP@C-dot hybrid system may improve the detection and monitoring of cell health and disease due to its dual-modal imaging capability. Furthermore, they could be used for supervising controlled release of therapeutic agents, tailored for enhanced treatment efficacy. This study demonstrates the potential of C-dot-conjugated AuNPs as a multifunctional tool with inherent control mechanisms for the next-generation cellular analysis, drug administration, and diagnostic strategies.Article Citation - WoS: 1Citation - Scopus: 1Protein Quantification Via Lspr-Based Biosensor Platform Utilizing Chrono-Growth for Enhanced Sensitivity(Elsevier, 2024) Sozmen, A. B.; Arslan-Yildiz, A.; 01. Izmir Institute of TechnologyIn this study an enhancement methodology, which utilizes time dependent growth of immobilized gold nanoparticles (GNPs) for LSPR-based biosensor platform was developed. The chrono-growth methodology was used for protein analysis and quantification. The method consisted GNP immobilization onto well-plates, GNP chronogrowth, and antibody functionalization. Success of each step was verified by UV-Vis spectrum measurement. Afterwards, the biosensor platform was tested to determine its characteristics. Bovine Serum Albumin (BSA) was chosen to be used as a model protein and an LoD value of 0.344 mu M and a dynamic detection range of 1 to 1000 mu M was calculated. The results were acquired within 30 min. Developed platform provides simple and rapid detection of the protein.Review Citation - WoS: 69Nanoparticle-Protein Corona Complex: Understanding Multiple Interactions Between Environmental Factors, Corona Formation, and Biological Activity(TAYLOR & FRANCIS LTD, 2021) Tomak, Aysel; Öksel Karakuş, Ceyda; Çesmeli, Selin; Öksel Karakuş, Ceyda; Hanoglu, Bercem D.; Winkler, David; Oksel Karakus, Ceyda; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe surfaces of pristine nanoparticles become rapidly coated by proteins in biological fluids, forming the so-called protein corona. The corona modifies key physicochemical characteristics of nanoparticle surfaces that modulate its biological and pharmacokinetic activity, biodistribution, and safety. In the two decades since the protein corona was identified, the importance of nanoparticles surface properties in regulating biological responses have been recognized. However, there is still a lack of clarity about the relationships between physiological conditions and corona composition over time, and how this controls biological activities/interactions. Here we review recent progress in characterizing the structure and composition of protein corona as a function of biological fluid and time. We summarize the influence of nanoparticle characteristics on protein corona composition and discuss the relevance of protein corona to the biological activity and fate of nanoparticles. The aim is to provide a critical summary of the key factors that affect protein corona formation (e.g. characteristics of nanoparticles and biological environment) and how the corona modulates biological activity, cellular uptake, biodistribution, and drug delivery. In addition to a discussion on the importance of the characterization of protein corona adsorbed on nanoparticle surfaces under conditions that mimic relevant physiological environment, we discuss the unresolved technical issues related to the characterization of nanoparticle-protein corona complexes during their journey in the body. Lastly, the paper offers a perspective on how the existing nanomaterial toxicity data obtained from in vitro studies should be reconsidered in the light of the presence of a protein corona, and how recent advances in fields, such as proteomics and machine learning can be integrated into the quantitative analysis of protein corona components.