WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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Access Right: Closed AccessAuthor: search.filters.author.Bulmuş, Volga

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  • Article
    Citation - WoS: 111
    Stabilization of Magnetic Iron Oxide Nanoparticles in Biological Media by Fetal Bovine Serum (fbs)
    (American Chemical Society, 2011) Wiogo, Hilda T. R.; Lim, May; Bulmuş, Volga; Yun, Jimmy; Amal, Rose
    A facile method of stabilizing magnetic iron oxide nanoparticles (MNPs) in biological media (RPMI-1640) via surface modification with fetal bovine scrum (FBS) is presented herein. Dynamic light scattering (DLS) shows that the size of the MNP aggregates can be maintained at 190 +/- 2 nm for up to 16 h in an RPMI 1640 culture medium containing >= 4 vol % FBS. Under transmission electron microscopy (TEM), a layer of protein coating is observed to cover the MNP surface following treatment with FBS. The adsorption of proteins is further confirmed by X-ray photoelectron spectroscopy (XPS). Gel electrophoresis and LC-MS/MS studies reveal that complement factor I-I, antithrombin, complement factor I, alpha-1-antiproteinase, and apolipoprotein E are the proteins most strongly attached to the surface of all MNP. These surface-adsorbed proteins serve as a linker that aids the adsorption of other serum proteins, such as albumin, which otherwise adsorb poorly onto MNPs. The size stability of FBS-treated MNPs in biological media is attributed to the secondary adsorbed proteins, and the size stability in biological media can be maintained only when both the surface-adsorbed proteins and the secondary adsorbed proteins are present on the particle's surface.
  • Conference Object
    Raft-Synthesis of Cholesterol Conjugated Polymers as Potential Therapeutic Carriers
    (American Chemical Society, 2011) Sevimli, Sema; İnci, Fatih; Bulmuş, Volga
    [No abstract available]
  • Article
    Citation - WoS: 34
    Doxorubicin Conjugated, Crosslinked, Pegylated Particles Prepared Via One-Pot Thiol-Ene Modification of a Homopolymer Scaffold: Synthesis and in Vitro Evaluation
    (Royal Society of Chemistry, 2011) Wong, Lingjiun; Kavallaris, Maria; Bulmuş, Volga
    Doxorubicin (Dox)-conjugated, poly(ethylene glycol) (PEG) shielded, reversibly crosslinked particles were prepared by a one-pot thiol-ene reaction from a RAFT-synthesized well-defined homopolymer scaffold, poly(pyridyldisulfide ethylmethacrylate) (PPDSM). Dox and PEG modified with maleimide end-groups (mal-Dox and mal-PEG), were covalently attached in one pot to free thiol groups of PPDSM (M-n = 8900 g mol(-1) and PDI = 1.18) in the presence of a disulfide reducing agent. similar to 50% of the total pyridyldisulfide units were conjugated with Dox and PEG (with an equal mol ratio). Particles with an average hydrodynamic diameter of 192 +/- 28 nm were observed to form after conjugation. Incubation of these particles with a disulfide reducing agent resulted in the disassociation of the particles. The release of Dox from the particles was pH dependent. The Dox-conjugated PEGylated particles (with a Dox content of 8 wt%) inhibited the viability of human cervical carcinoma cells (HeLa) with an IC50 value of 8 X 10(-7) M, determined by an Alamar Blue assay, while the IC50 of free Dox was 1 X 10(-7) M. The fluorescence microscopy analyses of the HeLa cells after incubation with the particles for varying times showed that the Dox carried by the particles is taken up efficiently by the cells.