WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7150

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Author: search.filters.author.Tomak, AyselSubject: search.filters.subject.Nanomaterials

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  • Article
    Proliferative Effects and Cellular Uptake of Ceramic Nanoparticles in Cancer and Normal Cells
    (Univ Chemistry & Technology, Prague, 2024) Cesmeli, Selin; Tomak, Aysel; Winkler, David A.; Karakus, Ceyda Oksel
    The high biocompatibility, wear resistance, and high surface area-to-volume ratios of calcium phosphate (CaP) nanoparticles make them materials of great interest for a very broad range of medical applications, such as dentistry, drug delivery, biomedical imaging, gene transfection and silencing, biomedical imaging, immunisation, and bone substitution. While their use as an enamel remineralisation agent, a bone substitution material, an implant coating, and drug/gene delivery agents is widely approved by the regulating bodies, insufficient attention has been paid to the interactions of CaP-based nanoparticles with cells and organs once in the bloodstream and distributed through the body. Here, three different CaP-based nanoparticles (CP: calcium phosphate, TCP: tricalcium phosphate, and HAp: hydroxyapatite) were examined for the proliferative effects, oxidative damage potential, and cellular uptake in the human embryonic kidney (HEK293) and pancreatic cancer (Panc-1) cell lines. The physicochemical properties of the nanoparticles were characterised by Teller analysis, and X-ray diffraction spectroscopy. Maximum proliferative effects were generated by 400 mu g center dot ml-1 TCP (220 %) in HEK293 cells. Interestingly, although CP nanoparticles had the highest reactive oxygen species formation capacity in the HEK293 cells, they exhibited the lowest proliferative effects and a relatively low internalisation rate, suggesting a minimal correlation between the cellular uptake level and oxidative potential.
  • Article
    Citation - WoS: 69
    Citation - Scopus: 73
    Nanoparticle-Protein Corona Complex: Understanding Multiple Interactions Between Environmental Factors, Corona Formation, and Biological Activity
    (Taylor & Francis, 2021) Öksel Karakuş, Ceyda; Tomak, Aysel; Çeşmeli, Selin; Hanoğlu, Berçem Dilan; Winkler, David
    The surfaces of pristine nanoparticles become rapidly coated by proteins in biological fluids, forming the so-called protein corona. The corona modifies key physicochemical characteristics of nanoparticle surfaces that modulate its biological and pharmacokinetic activity, biodistribution, and safety. In the two decades since the protein corona was identified, the importance of nano particles surface properties in regulating biological responses have been recognized. However, there is still a lack of clarity about the relationships between physiological conditions and cor ona composition over time, and how this controls biological activities/interactions. Here we review recent progress in characterizing the structure and composition of protein corona as a function of biological fluid and time. We summarize the influence of nanoparticle characteristics on protein corona composition and discuss the relevance of protein corona to the biological activity and fate of nanoparticles. The aim is to provide a critical summary of the key factors that affect protein corona formation (e.g. characteristics of nanoparticles and biological environ ment) and how the corona modulates biological activity, cellular uptake, biodistribution, and drug delivery. In addition to a discussion on the importance of the characterization of protein corona adsorbed on nanoparticle surfaces under conditions that mimic relevant physiological environment, we discuss the unresolved technical issues related to the characterization of nano particle-protein corona complexes during their journey in the body. Lastly, the paper offers a perspective on how the existing nanomaterial toxicity data obtained from in vitro studies should be reconsidered in the light of the presence of a protein corona, and how recent advances in fields, such as proteomics and machine learning can be integrated into the quantitative analysis of protein corona components.