WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7150

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Department: search.filters.department.İzmir Institute of TechnologySubject: search.filters.subject.Bioinformatics

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  • Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Effect of Mirna Administration on Non-Small Cell Lung Cancer Cells Studied by Cellular Viability Assay and Atr-Ftir Spectroscopy Combined With Multivariate Data-Analysis
    (Elsevier, 2025) Dagdeviren, Melih; Guler, Gunnur; Guler, Egemen Erdem; Un, Cemal; Karabay-Yavasoglu, Nefise Ulku
    MicroRNAs (miRNAs), small non-coding RNAs, play a significant role in the regulation of gene expression by various mechanisms. Some miRNAs such as hsa-miR-145 (mir145), hsa-let-7a-1 (let7), hsa-miR-155 (mir155), and hsa-miR-29b (mir29b) are expressed at low levels in cancers and associated with proliferation, metastasis, invasion and apoptosis. In the current study, we aimed to investigate the effect of selected synthetic miRNAs and their combinations on the non-small cell lung cancer (NSCLC) cells (A549) by following the cell viability profile and alterations in the cellular biomolecules with biophysical features. After administration of commercial miRNAs and their various combinations to A549 cell line, each group was analyzed with cell viability assay and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with unsupervised multivariate analysis. Bioinformatics analysis was also performed to detect and to classify the target human genes obtained from the mirDB database. According to the cell viability results, the "mir29b + let7" combination and "mir155" significantly decreased the cancer cell viability whereas the "mir145 + mir29b" and "mir155 + mir145" combinations dramatically increased the cancer cell viability when compared to the control cells. The FTIR data revealed that administration of the "mir155", "mir29b + let7 + mir155", and "mir29b + let7" combinations caused a decrease in the contents of proteins, lipids and nucleic acids in A549 cells. This study suggests that those miRNA combinations might be potential targets for vaccines or miRNA-based therapies that can restore the miRNA activity and thus should be further evaluated to combat lung cancer with miRNA technology.
  • Review
    Citation - WoS: 5
    Scientists Without Borders: Lessons From Ukraine [2]
    (Oxford Univ Press, 2023) Wolfsberger, Walter; Chhugani, Karishma; Shchubelka, Khrystyna; Frolova, Alina; Salyha, Yuriy; Zlenko, Oksana; Arych, Mykhailo
    Conflicts and natural disasters affect entire populations of the countries involved and, in addition to the thousands of lives destroyed, have a substantial negative impact on the scientific advances these countries provide. The unprovoked invasion of Ukraine by Russia, the devastating earthquake in Turkey and Syria, and the ongoing conflicts in the Middle East are just a few examples. Millions of people have been killed or displaced, their futures uncertain. These events have resulted in extensive infrastructure collapse, with loss of electricity, transportation, and access to services. Schools, universities, and research centers have been destroyed along with decades' worth of data, samples, and findings. Scholars in disaster areas face short- and long-term problems in terms of what they can accomplish now for obtaining grants and for employment in the long run. In our interconnected world, conflicts and disasters are no longer a local problem but have wide-ranging impacts on the entire world, both now and in the future. Here, we focus on the current and ongoing impact of war on the scientific community within Ukraine and from this draw lessons that can be applied to all affected countries where scientists at risk are facing hardship. We present and classify examples of effective and feasible mechanisms used to support researchers in countries facing hardship and discuss how these can be implemented with help from the international scientific community and what more is desperately needed. Reaching out, providing accessible training opportunities, and developing collaborations should increase inclusion and connectivity, support scientific advancements within affected communities, and expedite postwar and disaster recovery.