WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Review Citation - WoS: 17Citation - Scopus: 16Engineering Periodontal Tissue Interfaces Using Multiphasic Scaffolds and Membranes for Guided Bone and Tissue Regeneration(Elsevier, 2024) Özkendir, Özge; Karaca, İlayda; Çullu, Selin; Yaşar, Hüsniye Nur,; Erdoğan, Oğulcan; Dikici, Serkan; Dikici, Betul AldemirPeriodontal diseases are one of the greatest healthcare burdens worldwide. The periodontal tissue compartment is an anatomical tissue interface formed from the periodontal ligament, gingiva, cementum, and bone. This multifaceted composition makes tissue engineering strategies challenging to develop due to the interface of hard and soft tissues requiring multiphase scaffolds to recreate the native tissue architecture. Multilayer constructs can better mimic tissue interfaces due to the individually tuneable layers. They have different characteristics in each layer, with modulation of mechanical properties, material type, porosity, pore size, morphology, degradation properties, and drug-releasing profile all possible. The greatest challenge of multilayer constructs is to mechanically integrate consecutive layers to avoid delamination, especially when using multiple manufacturing processes. Here, we review the development of multilayer scaffolds that aim to recapitulate native periodontal tissue interfaces in terms of physical, chemical, and biological characteristics. Important properties of multiphasic biodegradable scaffolds are highlighted and summarised, with design requirements, biomaterials, and fabrication methods, as well as post-treatment and drug/growth factor incorporation discussed.Article Citation - WoS: 4Citation - Scopus: 4Optimizing the Dispersion of Calcium Phosphate Nanoparticles for Cellular Studies Using Statistical Design of Experiments(Elsevier, 2023) Önder, Anıl Can; Tomak, Aysel; Öksel Karakuş, CeydaThe in vitro experimentation of ceramic nanoparticles often requires their dispersion in liquid media without causing particle clumps or deteriorating sample integrity. However, the dispersion of nanoparticles using the available protocols rarely leads to stable and uniform dispersions which, in turn, raises concerns about the validity, repeatability and comparability of the findings observed in vitro. Moreover, the ability to control the final dispersion quality of ceramic nanoparticles is an essential step to obtaining optimized nanoceramic materials with desired functionality and to enhancing their performance in subsequent applications. While the need to have a comprehensive guideline for the dispersion of nanoparticles has led to several published documents and protocols, the dispersion methodology of ceramic nanoparticles and the relative contribution of the experimental parameters to the quality of resulting dispersion are still not clear. Here, we employed the statistical design of experiment (DoE) approach to systematically assess the magnitude and source of variation in dispersion quality of two different ceramic nanoparticles, hydroxyapatite and tricalcium phosphate. Using the first-order Plackett-Burman Design (PBD), nanoparticle concentration, pH and the presence of an additive were identified as the most critical factors influencing the resulting hydrodynamic size and zeta potential of the ceramic nanoparticles. Optimization using a second-order Central Composite Design (CCD) yielded a set of quadratic regression equations that were used to predict the hydrodynamic size or zeta potential of ceramic nanoparticles with high accuracy (R2, 0.88–0.92). The results of PBD screening and CCD optimization experiments were employed to prepare nanoparticle dispersions of different quality, which were then used to compare the effect of aggregation on the viability of human osteosarcoma (SaOS-2) cells. Overall, the results of this study provided insight into the role that various experimental parameters play in the colloidal stability and dispersion of ceramic nanoparticles. © 2023Article Citation - WoS: 7Citation - Scopus: 7Neo-Clerodanes From Teucrium Divaricatum Subsp. Divaricatum and Their Biological Activity Assessment(Elsevier, 2023) Aydoğan, Fadime; Ali, Zülfiqar; Zülfiqar, Fazila; Karaalp, Canan; Khan, Ikhlas A.; Bedir, ErdalFifteen neo-clerodane diterpenoids (1–15), including two undescribed glycosides, teudivaricosides A (1) and B (2), together with a known iridoid glycoside (16) and a phenylpropanoid glycoside (17) from the whole plant of Teucrium divaricatum subsp. divaricatum were isolated. Their structures were determined by spectral data analysis including 1D and 2D NMR and HRESIMS. Neo-clerodane diterpenoids were evaluated for their anti-inflammatory, and antimicrobial activities. None of them showed significant antimicrobial activity against various bacterial and fungal strains (up to 20 µg/mL). All tested compounds were inactive up to the highest tested concentration of 50 µM on iNOS inhibitory activity.Article Citation - WoS: 15Citation - Scopus: 16Electromechanical Rt-Lamp Device for Portable Sars-Cov Detection(Elsevier, 2023) Tarım, Ergün Alperay; Öksüz, Cemre; Karakuzu, Betül; Appak, Özgür; Sayıner, Ayça Arzu; Tekin, Hüseyin CumhurRapid point-of-care tests for infectious diseases are essential, especially in pandemic conditions. We have developed a point-of-care electromechanical device to detect SARS-CoV-2 viral RNA using the reverse-transcription loop-mediated isothermal amplification (RT-LAMP) principle. The developed device can detect SARS-CoV-2 viral RNA down to 103 copies/mL and from a low amount of sample volumes (2 μL) in less than an hour of standalone operation without the need for professional labor and equipment. Integrated Peltier elements in the device keep the sample at a constant temperature, and an integrated camera allows automated monitoring of LAMP reaction in a stirring sample by using colorimetric analysis of unfocused sample images in the hue/saturation/value color space. This palm-fitting, portable and low-cost device does not require a fully focused sample image for analysis, and the operation could be stopped automatically through image analysis when the positive test results are obtained. Hence, viral infections can be detected with the portable device produced without the need for long, expensive, and labor-intensive tests and equipment, which can make the viral tests disseminated at the point-of-care.Article Citation - WoS: 6Citation - Scopus: 7Designing Robust Xylan/Chitosan Composite Shells Around Drug-Loaded Msns: Stability in Upper Git and Degradation in the Colon Microbiota(Elsevier, 2023) Zeybek, Nüket; Büyükkileci, Ali Oğuz; Güleç, Şükrü; Polat, Mehmet; Polat, Hürriyetong residence times, near-neutral pH values, and release triggered by the enzymatic action of the resident microbiota offer unique opportunities for improved drug delivery in the colon. The fact that a delivery agent must also pass through the complete GI tract without degradation presents a challenge due to widely changing pH conditions. In this study, a promising colon-targeted drug delivery system was composed of a xylan/chitosan composite shell formed on curcumin-loaded mesoporous silica nanoparticles (MSNs). A novel synthesis approach was employed to facilitate precipitation of negatively charged xylan on negatively charged MSNs by concurrent chitosan polymerization. Curcumin-loaded xylan/chitosan-coated MSNs (C-MSNs) were determined to contain nearly 42% xylan by the inclusion of chitosan in a one-to-one ratio with xylan. The xylan/chitosan composite shell demonstrated excellent stability in the acidic upper GI tract. The hydrolysis of glycosidic bonds by resident microbiota was the triggering mechanism for xylan degradation and curcumin release in the colon. The presence of xylan has the further benefit of increasing the number of beneficial bacteria and improving short-chain fatty acid production for improved colon health.Conference Object Computational Nanotoxicology: a Case Study With Silver and Zinc Nanomaterials(Elsevier, 2022) Bilgi, Eyüp; Öksel Karakuş, CeydaNanomaterials (NMs) have been the focus of basic and applied research for more than two decades. According to the updated consumer materials inventory, over 1800 commercial NMs have taken their place in the market, 42% of which are in health and wellness category1. The widespread use of NMs in health-related products made not only the human exposure to the (residues of) NMs inevitable but also the long-recognized concerns over their safety a priority. Despite this pressing need, more than 70% of commercially available nano-containing products do not include sufficient information about their physicochemical and/or toxicological characteristics.Conference Object Citation - WoS: 1Green Synthesis of Nanostructured Bioactive Glass for Dental Applications(Elsevier, 2022) Tüncer, Melisa; Yücesoy, Deniz Tanıl; Öksel Karakuş, CeydaCalcium sodium phosphosilicate (known as bioactive glass) is a biomaterial commonly used in dental care products and bone tissue engineering applications due to biocompatibility, bone-forming and dentin sensitivity reduction capability. Bioactive 45S5 glass, so-called NovaMin, comprises of 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5 (wt%). It is traditionally synthesized by wet chemical methods such as melt-quenching and sol-gel which requires high temperature heating and the use of a strong acid catalyst, which brings into the question of the possibility of introducing toxic acid residues into the final product. Therefore, there is a clear need to develop environmental-friendly bioactive glass synthesis methods or to modify existing ones in a way to uplift their environmental friendliness.Conference Object Comparative Study of the Cytotoxicity of Hydroxyapatite, Tricalcium Phosphate and Calcium Phosphate Nanomaterials on Panc-1 and Hek293 Cell Line(Elsevier, 2022) Çeşmeli, Selin; Öksel Karakuş, CeydaCalcium phosphate-based bioceramic nanoparticles have been actively used in a range of therapeutic applications. Although they are mostly considered as biocompatible materials, the circulation of nanoparticles in the bloodstream raise further questions as to what degree of cellular damage they are capable of causing once carried out to vital organs such as kidney and pancreas. Therefore, there is a clear need to explore potential cellular damage induced by commercially used bioceramic nanoparticles such as hydroxyapatite (HAp), tricalcium phosphate (TCP) and calcium phosphate (CaP).Conference Object Development of Novel Nanotoxicity Assessment Method Utilizing 3d Printing System(Elsevier, 2022) Başlar, Muhammet Semih; Öksel Karakuş, Ceyda; Aldemir Dikici, BetülUnique physicochemical properties of nanomaterials (NMs) make them a material of choice in various applications but also raise concerns about their potential toxicity. While the commercial use of nano-enabled materials is growing rapidly, their interaction with biological systems and environment are not yet fully understood [1, 2]. Traditionally, toxicity of nano-sized materials are assessed by 2D cell culture models due to their time and cost-related advantages but their simplicity often comes at the cost of accuracy. While these methods are considered as the first step in toxicological assessment of both nanosized and bulk-form materials, they fall short in mimicking the complexity of in vivo physiological environments.Article Citation - WoS: 14Citation - Scopus: 16Synergistic Effect of Type and Concentration of Surfactant and Diluting Solvent on the Morphology of Emulsion Templated Matrices Developed as Tissue Engineering Scaffolds(Elsevier, 2022) Claeyssens, Frederik; Aldemir Dikici, Betül; Dikici, SerkanEmulsion templating is an advantageous route for the fabrication of tissue engineering scaffolds. Emulsions are mostly stabilised using surfactants, and the performances of the surfactants depend on various parameters such as emulsification temperature and the presence of the electrolytes. In this study, we suggest that diluting solvent type also has a dramatic impact on the efficiency of the surfactant and morphology of the polymerised emulsions. For this, morphologies of polycaprolactone methacrylate-based polymerised emulsions, which are designed for tissue engineering applications and in vitro biocompatibilities, were shown by our group, prepared using four different surfactants, and three different solvents were investigated. Results showed that the diluting solvent used in the emulsion composition has a strong impact on the performance of the surfactant and consequently on the morphology of polymerised emulsions. Increasing surfactant concentration and diluting solvent volume have an opposite impact on the characteristics of emulsions. Scaffolds with average pore sizes changing from 10 to 78 μm could be fabricated. Establishing these relations enables us to have control over the overall morphology of polymerised emulsions and precisely engineer them for specific tissue engineering applications by tuning solvent and surfactant type and concentration.