WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7150
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Article Citation - WoS: 13Citation - Scopus: 13Multi-Scale Benchtop 1h Nmr Spectroscopy for Milk Analysis(Academic Press, 2021) Söyler, Alper; Çıkrıkçı, Sevil; Çavdaroğlu, Çağrı; Bouillaud, Dylan; Farjon, Jonathan; Giraudeau, Patrick; Öztop, Mecit H.Benchtop NMR systems offers various advantages such as being easy to use, not requiring constant maintenance and being available at affordable prices. In this study, multiple aspects of benchtop NMR spectroscopy were explored to analyze milk in an industrial context, either regarding the quality of production or regarding the differentiation of the final product. The first part focuses on the production conditions of lactose hydrolysis in milk and quantitative online NMR spectroscopy was adapted to follow lactose hydrolysis in milk in continuous flow mode. The second part focuses on differentiating milk samples having different properties. 36 milk samples from France and Turkey were analysed and glycerol, fat and sugar contents were measured from the NMR spectra. Combination of spectroscopic data with a proposed Artificial Neural Network model enabled to classify milk of different origins and different properties. This study shows that benchtop NMR spectroscopy is a versatile non-destructive control method that can help controlling milk quality both during and after production. © 2020 Elsevier LtdArticle Citation - WoS: 49Citation - Scopus: 55Effects of Nisin and Lysozyme on Growth Inhibition and Biofilm Formation Capacity of Staphylococcus Aureus Strains Isolated From Raw Milk and Cheese Samples(International Association for Food Protection, 2012) Sudağıdan, Mert; Yemenicioğlu, AhmetEffects of nisin and lysozyme on growth inhibition and biofilm formation capacity of 25 Staphylococcus aureus strains isolated from raw milk (13 strains) and cheese (12 strains) were studied. Nisin was tested at concentrations between 0.5 and 25 μg/ ml; the growth of all strains was inhibited at 25 μg/ml, but the resistances of strains showed a great variation at lower nisin concentrations. In contrast, lysozyme tested at concentrations up to 5.0 mg/ml showed no inhibition on the growth of strains. Nisin used at the growth inhibitory concentration prevented the biofilm formation of strains, but strains continued biofilm formation at subinhibitory nisin concentrations. Lysozyme did not affect the biofilm formation of 19 of the strains, but it caused a considerable activation in the biofilm formation capacity of six strains. Twelve of the strains contained both biofilm-related protease genes (sspA, sspB, and aur) and active proteases; eight of these strains were nisin resistant. These results suggest a potential risk of S. aureus growth and biofilm formation when lysozyme is used in the biopreservation of dairy products. Nisin can be used to control growth and biofilm formation of foodborne S. aureus, unless resistance against this biopreservative develops. Copyright ©, International Association for Food Protection.Article Citation - WoS: 8Citation - Scopus: 8Differential Scanning Calorimetry as a Tool To Detect Antibiotic Residues in Ultra High Temperature Whole Milk(John Wiley and Sons Inc., 2009) Yıldız, Özge; Ünlütürk, SevcanDetection of penicillin G, ampicillin and tetracycline in ultra high temperature whole milk was studied by differential scanning calorimetry (DSC). Thermal parameters including the heat of fusion, the evaporation temperature, the heat of evaporation and the melting temperature obtained from DSC analysis were used to characterise thermal behaviour of antibiotic free milk samples and milk samples fortified with Penicillin G, Ampicillin and Tetracycline. DSC curves of these antibiotics at selected concentrations (0, 2, 4, 8 ppb for Penicillin G and Ampicillin; 0, 100, 250, 500 ppb for Tetracycline) show big endothermic peaks in the temperature range of -30 °C and 200 °C. It was concluded that the antibiotic concentration had a significant effect on the thermal parameters at a 95% confidence level. The differences between the melting temperatures and the peak areas in heat flow curves provided a basis for detection of antibiotic residues in UHT whole milk.Article Citation - WoS: 22Citation - Scopus: 24Prolactin Receptor Gene Expression in Rat Splenocytes and Thymocytes From Birth To Adulthood(Elsevier Ltd., 1996) Güneş, Hatice; Mastro, Andrea M.In vivo and in vitro studies have indicated that the anterior pituitary hormone prolactin (PRL) is an immunoregulator and functions in the development of the neonatal immune system. In this study, prolactin receptor (PRL-R) expression from birth to adulthood as well as the effect of milk ingestion on the PRL-R expression were examined in splenocytes and thymocytes of neonatal rats. Three approaches were taken to measure PRL-R expression: (i) polymerase chain reaction (RT-PCR); (ii) antibody to PRL-R and Western blotting; (iii) antibody to PRL-R and flow cytometry. RT-PCR analysis revealed the short and long form of PRL-R mRNA in both spleen and thymus at every age tested. However, the long form of PRL-R mRNA was always more abundant than that of the short form. In addition, antipeptide antibody against the long form of PRL-R recognized 84 and 42 kD proteins in the spleen, but only the 84 kD protein in the thymus. A monoclonal antibody U6 recognized 38 and 40 kD proteins in both the spleen and thymus. Although the mRNA level of PRL-R was relatively low at birth and increased with age in both the spleen and thymus, the levels of protein bands detected with both antibodies correlated with development in the spleen; whereas the levels remained steady in the thymus. Therefore, we concluded that the expression of PRL-R at the protein level is developmentally regulated in the spleen but not in the thymus. Finally, milk ingestion in the first seven hours decreased the percentage of cells expressing cell surface PRL-R, suggesting that milk-borne PRL may have a direct effect on lymphocytes.