TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7149
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Article Determination of Resistance Levels To Clavibacter Michiganensis Subsp. Michiganensis in Some Solanum Species(Ege Tarımsal Araştırma Enstitüsü, 2022) Frary, Anne; Şanver, Utku; Akköse Baytar, Asena; Özaktan, Hatice; Doğanlar, SamiClavibacter michiganensis subsp. michiganensis (Cmm), is a devastating bacterial disease agent causing bacterial wilt and canker in tomatoes. There is no definitive solution to prevent yield losses by Cmm in tomatoes. Moreover, there is currently no commercially successful Cmm resistant tomato cultivar on the market. Therefore, we aimed to determine the tolerance level of some tomato accessions to Cmm in the present study. For this purpose, we screened seven tomato accessions representing four species (Solanum arcanum, S. habrochaites, S. pennellii, and S. peruvianum) from Peru, Ecuador, and Mexico against the highly virulent isolates Cmm-244 and Cmm-9. A root immersion method was used to identify new sources of resistance to this important disease. Two accessions, S. habrochaites LA1777, and S. arcanum LA2157 were found to be moderate and highly tolerant, respectively, and could serve as tolerance resources for tomato breeding in Türkiye. These materials can also be investigated more extensively to determine their intrinsic Cmm tolerance mechanism.Article Citation - WoS: 25Citation - Scopus: 25Application of Est-Ssrs To Examine Genetic Diversity in Eggplant and Its Close Relatives(TUBITAK, 2011) Tümbilen, Yeliz; Frary, Anne; Daunay, Marie Christine; Doğanlar, SamiWithin the genus Solanum, the term 'eggplant' encompasses several cultivated species that are used for food and, to a lesser extent, for medicine. Th e use of one common name to describe more than one species and the existence of many related wild species have led to taxonomic confusion which, in turn, have complicated analyses of evolutionary relationships and genetic diversity within this groups of species. A further challenge for eggplant research is that, despite the fact that the use of molecular markers for phylogenetic studies is well-established, very few studies have described the development of new markers for eggplant. In our work, genic microsatellite (SSR) markers were identified from an expressed sequence tag library of S. melongena and used for analysis of 47 accessions of eggplant and closely related species. Th e markers had very good polymorphism in the 18 species tested including 8 S. melongena accessions. Moreover, genetic analysis performed with these markers showed concordance with previous research and knowledge of eggplant domestication. Th ese markers are expected to be a valuable resource for studies of genetic relationships, fingerprinting, and gene mapping in eggplant.Article Citation - WoS: 9Citation - Scopus: 13Quantitative Trait Loci (qtl) Analysis for Antioxidant and Agronomically Important Traits in Tomato (lycopersicon Esculentum)(Türkiye Klinikleri Journal of Medical Sciences, 2011) Ökmen, Bilal; Şığva, Hasan Özgür; Gürbüz, Nergiz; Ülger, Mehmet; Frary, Anne; Doğanlar, SamiTomato is one of the most widely produced and consumed vegetable crops worldwide. Plant breeders have usually focused on improvement of horticulturally important traits such as yield, fruit size, shape and colour. With increased attention on human health, however, plant breeders also consider the improvement of health-related traits of fruits and vegetables such as antioxidant characters. In the present study, genes controlling both health-related and horticulturally important traits were mapped in the tomato genome using 152 Lycopersicon hirsutum BC2F2 individuals. For this aim, all plants were phenotypically and genotypically characterised and a total of 75 QTLs were identified for all traits. Of the 75 QTLs, 28 were identified for 5 antioxidant traits including total water soluble antioxidant capacity, vitamin C, total phenolics, total flavonoids, and lycopene contents, and 47 QTLs were identified for 8 agronomic traits including fruit weight, external and internal fruit colour, fruit firmness, fruit shape, stem scar size, locule number, and wall thickness. Markers linked with these QTLs can be used in marker assisted selection (MAS) for improvement of elite tomato lines. © TÜBİTAK.Article Citation - Scopus: 2Identification of Potato Y Potyvirus (pvy°) Resistance in Wild and Cultivated Tomatoes(Türkiye Klinikleri Journal of Medical Sciences, 2009) Çelebi Toprak, Fevziye; Barutçu, Eminur; Frary, Anne; Doğanlar, SamiPotato Y potyvirus (PVY) is an important plant pathogen worldwide that infects and causes yield losses in the family Solanaceae including potato (Solarium tuberosum), pepper (Capsicum spp.), tomato (S. lycopersicum), and tobacco (Nicotiana tabacum). In this study, 20 different tomato accessions representing 6 different species were mechanically inoculated with PVY°. The plants were scored visually for symptoms and then tested for presence of the virus 2-4 weeks after inoculation by ELISA. The results were variable. Most wild species of tomato sustained PVY° replication in inoculated leaves. Some of the wild species showed an immune response, while some became systemically infected. Inoculation and analysis of F2 populations suggested that the resistance is controlled by a single recessive gene in different wild species.