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Citation - WoS: 12
Citation - Scopus: 15
Morphological and Molecular Identification of Pennate Diatoms Isolated From Urla, Izmir, Coast of the Aegean Sea
(TUBITAK, 2013) Kesici, Kutsal; Tüney, İnci; Sukatar, Atakan; Zeren, Doğuş; Güden, Mustafa
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as ITS (Internal Transcribed Spacer) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated four diatom samples from Coast of Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then, we amplified ITS regions using conventional polymerase chain reaction (PCR), sequenced the PCR products and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia sp., Achnanthes sp., Amphora sp. and Cylindrotheca sp. We believe that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.

TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection