Chemical Engineering / Kimya Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/14
Browse
4 results
Search Results
Research Project Kateşol-O-metiltransferaz (KOMT) enzim aktivitesinin bitkilerden elde edilen bazı alkaloitler ve fenollerle engellenmesi(2009) Bayraktar, OğuzBu çalışmada, üzerlik tohumu alkaloitleri ve zeytin yaprağı polifenollerinin KOMT tarafından katalizlenen metillenme reaksiyonunu engelleyebilme potansiyelleri araştırılmıştır. Özellikle, ekstraksiyon ve fraksiyonlama işlemleri ile saflıkları arttırılmaya çalışılmış harmin ve harmalin alkaloitlerinin ve oleuropein ve rutin polifenollerinin KOMT enzim inhibisyonu performansları, bu bileşiklerin standartları ve bilinen en iyi KOMT inhibitörü olan 3,5- dinitrokateşol ile kıyaslanmıştır. Bitki özütlerinin elde edilmesi ve fraksiyonlama işlemleri sırasında verimliliğe etki eden bir çok parametre YPSK analizlerine göre optimize edilmiştir. KOMT inhibisyon deneylerinde kullanılmak üzere elde edilmiş özüt ve fraksiyonların karakterizasyonu da yine YPSK analizleri ile gerçekleştirilmiştir.Research Project Yara örtüsü uygulamaları için eguisetum arvense özüyü yüklü biyomalzemelerin hazırlanması ve karakterizasyonu(2012) Bayraktar, OğuzYara, deride fiziksel, ısı kaynaklı veya fizyolojik bir etken ile oluşan tahribatı tanımlamak için kullanılmaktadır. Yara iyileşmesi farklı tip hücreler ve dokuyu oluşturan bileşenlerin, çevresel sinyallere verdikleri tepkiler ile ilerlemektedir. Yara iyileştirme terimi lokal farmasötik formülasyonlardan sentetik bandaj gibi geleneksel yara örtülerinden modern uygulamalar olan doku destek malzemelere kadar uzanan bir aralığı kapsamaktadır.Article Citation - WoS: 5Citation - Scopus: 8A Research on the Production of Silk Sericin Powders by Using Spray Drying Method(Ege Üniversitesi, 2009) Genç, Gözde; Bayraktar, Oğuz; Başal, GüldemetSericin protein is usually discarded as a waste in silk industry. Production of silk sericin powders, which can be incorporated into fibers used in textile industry, by spray drying method will enable value-added utilization of waste sericin. in this study aqueous sericin solutions were used as raw material for the production of dry powders using a lab-scale spray dryer. A linear regression analyses were employed, in addition to experimental design at two levels with three factors for the analysis of three responses: moisture content, particle type and agglomeration degree. The process factors were the drying air temperature (120°C and 160°C), the feed rate (1.25x10-4 and 2.5x10-4 dms -), and the concentration of sericin solutions of 10% and 30% (w/w) fed to the spray dryer. The three responses were analyzed statistically to determine the effective parameters and it was concluded that moisture content depended on three factors-drying air temperature being the dominant parameter. Particle size and shape depended mainly on feed rate and agglomeration depended on the moisture content of the product.Article Kinetic and Structural Characterization of Interaction Between Trypsin and Equisetum Arvense Extract(Türk Biyokimya Derneği, 2014) Uslu, Mehmet Emin; Bayraktar, Oğuz; Ceylan, ÇağatayObjective: In this study the inhibitory effect of E. arvense extract on trypsin activity and the effect of trypsin on E. arvense extract were studied. In addition the nature of the interaction between the extract and trypsin was investigated. Methods: The inhibitory effect ethanol extract of E. arvense on trypsin activity was determined using trypsin enzyme assay. The structural effects of the extract-trypsin interaction for the extract were analyzed by FTIR. Finally, the HPLC analyses were carried out to analyze the individual components of the extract and the supernatant and soluble precipitate phases. Results: E. arvense extract was found to decrease total percent activity of trypsin to 5% in 24 hour at 24 °C. FTIR analyses indicated that the interaction between trypsin and E. arvense extract caused changes in the structure and hydrogen bonding behavior and composition of the extract proteins. These interactions also caused the extract lipids to accumulate in the insoluble precipitate phase. Most of the phenolics remained in the supernatant phase enhancing the inactivation of trypsin. However, the precipitated compounds were shown to be of apolar in nature as shown in the HPLC chromatograms. Conclusion: The methods that were used showed that the high phenolic content of E. arvense was the main reason for the inhibition of trypsin enzyme activity by denaturing the enzyme.