Chemical Engineering / Kimya Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/14

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Author: search.filters.author.Davis, Thomas P.Subject: search.filters.subject.RAFT polymerization

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  • Article
    Citation - WoS: 46
    Citation - Scopus: 46
    Conjugation of Sirna With Comb-Type Peg Enhances Serum Stability and Gene Silencing Efficiency
    (John Wiley and Sons Inc., 2011) Gunasekaran, Karthikeyan; Nguyen, Thi H.; Maynard, Heather D.; Davis, Thomas P.; Bulmuş, Volga
    A thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56± 5%-58± 3% of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77± 7%).