Chemical Engineering / Kimya Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/14
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Article Citation - WoS: 25Citation - Scopus: 29Antibacterial Properties of Silk Fibroin/Chitosan Blend Films Loaded With Plant Extract(Korean Fiber Society, 2010) Başal, Güldemet; Altıok, Duygu; Bayraktar, Oğuz; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe silk fibroin (SF)/chitosan (CHI) blend films were prepared by dissolving them in formic acid. The morphology of the films was examined by scanning electron microscopy (SEM). The roughness of the membranes was determined by atomic force microscopy (AFM). These films were treated with the extracts of Pistacia terebinthus, Pistacia lentiscus, and Hypericum empetrifolium. Folin-Ciocalteu assay was used to determine the amount of total phenols adsorbed on these blend films. The antibacterial properties of films were tested using disc diffusion and turbidity measurement methods against Escherichia coli and Staphylococcus epidermidis. The release of adsorbed phenolics from the film surface resulted in antibacterial properties. © The Korean Fiber Society and Springer Netherlands 2010.Article Citation - WoS: 42Citation - Scopus: 60Pistacia Terebinthus Extract: as a Potential Antioxidant, Antimicrobial and Possible Ss-Glucuronidase Inhibitor(Elsevier Ltd., 2010) Demirbüker Kavak, Dilek; Altıok, Evren; Ülkü, Semra; Ülkü, Semra; Demirbüker Kavak, Dilek; Bayraktar, Oğuz; 03.08. Department of Food Engineering; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyIn this study, inhibition of Escherichia coli glucuronidase (GUS) by a plant extract of Pistacia terebinthus was investigated. P. terebinthus (from Karaburun region in Turkey) leaf extract exhibits various bioactivities, such as antioxidant, antimicrobial and cytotoxic property because of its flavonoid, phenolic and alkaloid contents. HPLC analyses were performed to evaluate the composition of the extract and main flavonoid compounds such as luteolin, luteolin-7-glucoside and apigenin-7-glucoside were detected in extract. GUS activity and kinetic data of the GUS were obtained by measuring rate of production of resulting fluorescent 4-methylumbelliferone (4-MU) and phenolphthalein fluorometrically and spectrophotometrically, respectively. Plant leaves showed higher antioxidant capacity as 85 TEAC (trolox equivalent antioxidant capacity) value and higher antimicrobial activity towards Staphylococcus aureus. 92.4% GUS enzyme inhibition was achieved with 8.3 μg ml-1 crude extract concentration, where IC50 value was found as 2.1 (±0.2) μg ml-1. Kinetic studies by nonlinear regression revealed that inhibition type caused by plant extract was mixed type (noncompetitive and competitive together). Km and Vmax values were 96.94 ± 9.68 nM and 7.81 ± 0.16 nmol min-1 mg-1, respectively. Inhibition constant (αKi) was found as 7.00 ± 0.93 nM having α value 2.17 ± 0.93. Our results may provide useful information regarding the inhibition of microbial GUS activity, preventing deglucuronidation and reducing possible cancer risk by bioactive P. terebinthus crude extract. © 2010 Elsevier B.V. All rights reserved.
